Rashidi Hooman H, Xu Xiangdong, Wang Huan-You, Shafi Nelofar Q, Rameshkumar Karuna, Messer Karen, Smith Brian R, Rose Michal G
University of California San Diego School of Medicine & VA Medical Center San Diego, USA.
Int J Clin Exp Pathol. 2012;5(3):224-30. Epub 2012 Mar 25.
The diagnostic utility of flow cytometry in the evaluation of cytopenias and in the differential diagnosis of low-grade versus high-grade myelodysplastic syndrome (MDS) is not widely appreciated. In this report, we measured granulocyte CD10/control fluorescence ratio in 29 patients with MDS & chronic myelomonocytic leukemia (CMML) using peripheral blood (PB) flow cytometry (FC). We found a lower ratio in high-grade MDS and CMML (mean ratio of 2.2 ± 0.7) vs. low-grade MDS (3.65 ± 0.9) and 16 cytopenic controls without MDS (3.67 ± 0.65; p<0.001). The sensitivity and specificity of CD10 ratio <3 for the group that included the high risk MDS and CMML patients were 87.5% and 100%, respectively. Our data suggests that FC of PB may be helpful in the work-up of patients with cytopenias and in the differential diagnosis of low-grade vs. high-grade MDS.
流式细胞术在血细胞减少症评估以及低级别与高级别骨髓增生异常综合征（MDS）鉴别诊断中的诊断效用尚未得到广泛认可。在本报告中，我们使用外周血流式细胞术（FC）测量了29例MDS及慢性粒单核细胞白血病（CMML）患者的粒细胞CD10/对照荧光比率。我们发现，高级别MDS和CMML患者的比率较低（平均比率为2.2±0.7），而低级别MDS患者（3.65±0.9）以及16例无MDS的血细胞减少症对照患者（3.67±0.65；p<0.001）的比率较高。对于包括高危MDS和CMML患者的组，CD10比率<3的敏感性和特异性分别为87.5%和100%。我们的数据表明，外周血流式细胞术可能有助于血细胞减少症患者的检查以及低级别与高级别MDS的鉴别诊断。
