Ziegler Peter E, Wade Susan E, Schaaf Stephanie L, Chang Yung-Fu, Mohammed Hussni O
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
J Wildl Dis. 2007 Oct;43(4):586-96. doi: 10.7589/0090-3558-43.4.586.
The objective of this study was to assess the potential role that wildlife plays in environmental degradation of watersheds through the contamination of the water supply with zoonotic genotypes of Cryptosporidium. Cryptosporidium isolates recovered from wildlife in the New York City (NYC) watershed were examined to determine genotype using a polymerase chain reaction protocol targeting the 18-Small Subunit (SSU) rRNA locus. Seventy-seven DNA samples recovered from 12 wildlife host species captured in the NYC watershed were amplified and sequenced. Data on risk factors associated with the perpetuation of these genotypes also were collected and analyzed. Although many genotypes appeared to be host-specific, 38% of the samples examined were identified as Cryptosporidium parvum, indicating the presence of zoonotic Cryptosporidium. Adult animals were more likely to shed the zoonotic strains of Cryptosporidium spp. Animals captured in the fall and winter were more likely to be infected with C. parvum than those captured in spring and summer.
本研究的目的是评估野生动物通过水源被隐孢子虫人畜共患病基因型污染而在流域环境退化中所起的潜在作用。对从纽约市（NYC）流域野生动物中分离出的隐孢子虫进行检测，使用针对18 - 小亚基（SSU）rRNA基因座的聚合酶链反应方案来确定基因型。对从NYC流域捕获的12种野生动物宿主物种中获得的77个DNA样本进行扩增和测序。还收集并分析了与这些基因型持续存在相关的风险因素数据。尽管许多基因型似乎具有宿主特异性，但所检测样本中有38%被鉴定为微小隐孢子虫，这表明存在人畜共患的隐孢子虫。成年动物更有可能排出隐孢子虫属的人畜共患病菌株。秋季和冬季捕获的动物比春季和夏季捕获的动物更有可能感染微小隐孢子虫。
