Stark Ken D
The Laboratory of Nutritional and Nutraceutical Research, Department of Kinesiology, University of Waterloo, Waterloo, ON, Canada N2L 3G1.
Lipids. 2008 Jan;43(1):45-53. doi: 10.1007/s11745-007-3128-3. Epub 2007 Nov 6.
A blood biomarker of omega-3 fatty acid intake and tissue status could serve as a modifiable risk factor for cardiovascular disease. The percentage of omega-3 highly unsaturated fatty acid (HUFA > or = 20 carbons and > or =3 double bonds) in the total HUFA pool (the n-3 HUFA score) was examined as a potential blood biomarker of omega-3 fatty acids in tissues. The fatty acid composition of total lipid extracts (TLE) and phospholipid (PL) fractions were determined for plasma and erythrocytes samples of human subjects (n = 20) and the n-3 HUFA score and the sum of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were compared. Omega-3 fatty acids in blood and tissues of rats (n = 31) and pigs (n = 48) were also determined and the associations were compared. The n-3 HUFA score is more consistent across plasma and erythrocytes, with strong correlations between TLE and PL in plasma (r = 0.93) and erythrocytes (r = 0.94). The n-3 HUFA score was less variable and blood levels correlated strongly with various animal tissues. The n-3 HUFA score is a useful blood biomarker that does not require the isolation of the PL class thereby supporting high throughput analyses. The strength of association between the n-3 HUFA score and disease risk needs to be examined.
ω-3脂肪酸摄入量和组织状态的血液生物标志物可作为心血管疾病的一个可改变风险因素。将ω-3高度不饱和脂肪酸（HUFA≥20个碳原子且≥3个双键）在总HUFA库中的百分比（n-3 HUFA评分）作为组织中ω-3脂肪酸的潜在血液生物标志物进行检测。测定了人类受试者（n = 20）血浆和红细胞样本中总脂质提取物（TLE）和磷脂（PL）组分的脂肪酸组成，并比较了n-3 HUFA评分以及二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的总和。还测定了大鼠（n = 31）和猪（n = 48）血液和组织中的ω-3脂肪酸，并比较了相关性。n-3 HUFA评分在血浆和红细胞中更具一致性，血浆中TLE与PL之间（r = 0.93）以及红细胞中TLE与PL之间（r = 0.94）具有强相关性。n-3 HUFA评分变异性较小，血液水平与各种动物组织密切相关。n-3 HUFA评分是一种有用的血液生物标志物，不需要分离PL类别，因此支持高通量分析。n-3 HUFA评分与疾病风险之间的关联强度需要进行研究。
