Lin WeiWei, Chen Xue, Wang XiaoDong, Liu Jie, Gu XiaoSong
The Jiangsu Province Key Lab of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, China.
In Vitro Cell Dev Biol Anim. 2008 Jan-Feb;44(1-2):31-40. doi: 10.1007/s11626-007-9064-y. Epub 2007 Nov 6.
Bone marrow stromal cells (MSCs) have the capability of differentiating into mesenchymal and non-mesenchymal lineages. In this study, MSCs isolated from adult Sprague-Dawley rats were cultured to proliferation, followed by in vitro induction under specific conditions. The results demonstrated that MSCs were transdifferentiated into cells with the Schwann cell (SC) phenotypes according to their morphology and immunoreactivities to SC surface markers including S-100, glial fibrillary acidic protein (GFAP) and low-affinity nerve growth factor receptor (p75). Consequently, rat adult MSCs can be induced in vitro to differentiate into SC-like cells, thus developing an abundant and accessible SC reservoir to meet the requirements of constructing tissue engineered nerve grafts for peripheral nerve repair.
骨髓基质细胞（MSCs）具有分化为间充质和非间充质谱系的能力。在本研究中，从成年Sprague-Dawley大鼠分离出的MSCs培养至增殖状态，然后在特定条件下进行体外诱导。结果表明，根据其形态以及对包括S-100、胶质纤维酸性蛋白（GFAP）和低亲和力神经生长因子受体（p75）在内的雪旺细胞（SC）表面标志物的免疫反应性，MSCs转分化为具有SC表型的细胞。因此，成年大鼠MSCs可在体外被诱导分化为类SC细胞，从而形成丰富且易于获取的SC储备，以满足构建用于周围神经修复的组织工程神经移植物的需求。
