Feng J R, Chen X S, Yuan Z H, Zhang L J, Ci Z J, Liu X L, Zhang C Y
Horticultural Department, Shihezi University, Shihezi, Xinjiang 832003, China.
Mol Biol Rep. 2009 Feb;36(2):263-72. doi: 10.1007/s11033-007-9175-2. Epub 2007 Nov 7.
Expression of the S-RNase genes in the self-compatible (SC) apricot (Prunus armeniaca L.) cultivar Katy, the self-incompatible (SI) cultivar Xinshiji and their F(1) seedling was examined in this study. Three S-genotypes, S(9)Sc (Sc, self-compatibility S-gene absent from the style), S(8)S(9), and S(8)S(10), were obtained. Seedlings with S-RNase that migrated as a single band in gel electrophoresis were SC, despite high transcript abundance, and those with S-RNase that migrated as two bands were SI with high transcript abundance or SC with low transcript expression. S(8)-RNase was induced in SI cultivars only 24 h after self-pollination, indicating post-transcriptional regulation of S(8)-RNase in SI apricots. A Proteomic study showed that 35 protein spots were synthesized differently between SC and SI pistils. Fifteen of the 35 protein spots were identified; nine proteins, including receptor protein kinase-like protein, reversibly glycosylated polypeptide-2, and isoflavone reductase-like protein, were detected only in the SC pistils; while nine proteins, including actin 7, a putative serine/threonine kinase, and S-RNase, were detected only in the SI pistils. A mitochondrial NAD-dependent malate dehydrogenase and a probable elongation factor G were up-regulated, while heat shock cognate 70 was down-regulated in the SC pistils compared to those in the SI pistils. The results suggest that the proteins responsible for self-compatibility and self-incompatibility may be different.
本研究检测了自交亲和（SC）的杏（Prunus armeniaca L.）品种Katy、自交不亲和（SI）品种新世纪及其F(1)代幼苗中S-RNase基因的表达情况。获得了三种S基因型，即S(9)Sc（Sc，花柱中不存在自交亲和性S基因）、S(8)S(9)和S(8)S(10)。在凝胶电泳中迁移为单一带的含有S-RNase的幼苗为自交亲和型，尽管转录本丰度较高；而迁移为两条带的含有S-RNase的幼苗为自交不亲和型且转录本丰度较高，或者为自交亲和型但转录本表达量较低。S(8)-RNase仅在自花授粉后24小时在自交不亲和品种中被诱导，表明在自交不亲和的杏中S(8)-RNase存在转录后调控。一项蛋白质组学研究表明，自交亲和与自交不亲和的雌蕊中有35个蛋白点的合成存在差异。已鉴定出35个蛋白点中的15个；其中9种蛋白质，包括类受体蛋白激酶、可逆糖基化多肽-2和类异黄酮还原酶，仅在自交亲和的雌蕊中检测到；而另外9种蛋白质，包括肌动蛋白7、一种假定的丝氨酸/苏氨酸激酶和S-RNase，仅在自交不亲和的雌蕊中检测到。与自交不亲和的雌蕊相比，自交亲和的雌蕊中一种线粒体NAD依赖的苹果酸脱氢酶和一种可能的延伸因子G上调，而热休克同源蛋白70下调。结果表明，负责自交亲和与自交不亲和的蛋白质可能不同。
