通过基于表达荧光素酶的假病毒细胞内组装的检测方法，从自然感染的患者中检测人乳头瘤病毒31型中和抗体。
Detection of human papillomavirus type 31-neutralizing antibodies from naturally infected patients by an assay based on intracellular assembly of luciferase-expressing pseudovirions.
作者信息
Fleury Maxime J J, Touzé Antoine, de Sanjosé Silvia, Bosch F Xavier, Klaustermeiyer Joellen, Coursaget Pierre
机构信息
INSERM U618, Tours, France.
出版信息
Clin Vaccine Immunol. 2008 Jan;15(1):172-5. doi: 10.1128/CVI.00292-07. Epub 2007 Nov 7.
Abstract
The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay.
摘要
本研究的目的是基于携带荧光素酶的假病毒的产生，开发一种高度敏感的31型人乳头瘤病毒（HPV31）中和试验。在一组HPV31单克隆抗体以及有HPV31感染证据的女性中研究了针对HPV31的中和抗体。在酶联免疫吸附测定呈阳性的受试者中，78%检测到了中和抗体。
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