利用重组杆状病毒产生的重组人乳头瘤病毒16型L1衣壳，通过酶联免疫吸附测定法检测抗人乳头瘤病毒16型病毒体的抗体。
Detection of antibodies against human papillomavirus (HPV) type 16 virions by enzyme-linked immunosorbent assay using recombinant HPV 16 L1 capsids produced by recombinant baculovirus.
作者信息
Le Cann P, Touze A, Enogat N, Leboulleux D, Mougin C, Legrand M C, Calvet C, Afoutou J M, Coursaget P
机构信息
Service des Maladies Infectieuses, Hôpital de Fann, Dakar, Senegal.
出版信息
J Clin Microbiol. 1995 May;33(5):1380-2. doi: 10.1128/jcm.33.5.1380-1382.1995.
Abstract
The L1 major capsid protein of human papillomavirus type 16 (HPV-16) was expressed in Sf-21 insect cells with a recombinant baculovirus. Virus-like particles obtained were purified and used to develop an enzyme-linked immunosorbent assay for detection of anti-HPV-16 antibodies in sera from 76 women with evidence of genital HPV infection and 79 controls. HPV-16-infected individuals developed antibodies directed at HPV-16 virions since reactivity against recombinant HPV-16L1 capsids was observed in 50% of them compared with only 6% in the general adult population. However, some cross-reactivities with sera from women infected with others HPV types were observed.
摘要
人乳头瘤病毒16型（HPV - 16）的L1主要衣壳蛋白通过重组杆状病毒在Sf - 21昆虫细胞中表达。所获得的病毒样颗粒经过纯化，并用于开发一种酶联免疫吸附测定法，以检测76名有生殖器HPV感染证据的女性血清和79名对照中的抗HPV - 16抗体。HPV - 16感染个体产生了针对HPV - 16病毒体的抗体，因为在50%的感染者中观察到了与重组HPV - 16L1衣壳的反应性，而在普通成年人群中这一比例仅为6%。然而，也观察到了与其他HPV类型感染女性血清的一些交叉反应。
相似文献
1
Detection of antibodies against human papillomavirus (HPV) type 16 virions by enzyme-linked immunosorbent assay using recombinant HPV 16 L1 capsids produced by recombinant baculovirus.利用重组杆状病毒产生的重组人乳头瘤病毒16型L1衣壳，通过酶联免疫吸附测定法检测抗人乳头瘤病毒16型病毒体的抗体。
J Clin Microbiol. 1995 May;33(5):1380-2. doi: 10.1128/jcm.33.5.1380-1382.1995.
2
A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16.一种病毒样颗粒酶联免疫吸附测定法可检测出大多数感染人乳头瘤病毒16型的女性血清中的抗体。
J Natl Cancer Inst. 1994 Apr 6;86(7):494-9. doi: 10.1093/jnci/86.7.494.
3
Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles.人乳头瘤病毒11型L1蛋白在昆虫细胞中的表达：病毒样颗粒的体内和体外组装
J Virol. 1993 Apr;67(4):1936-44. doi: 10.1128/JVI.67.4.1936-1944.1993.
4
Production of recombinant virus-like particles from human papillomavirus types 6 and 11, and study of serological reactivities between HPV 6, 11, 16 and 45 by ELISA: implications for papillomavirus prevention and detection.人乳头瘤病毒6型和11型重组病毒样颗粒的产生，以及通过酶联免疫吸附测定法研究人乳头瘤病毒6型、11型、16型和45型之间的血清学反应：对乳头瘤病毒预防和检测的意义
FEMS Microbiol Lett. 1998 Mar 1;160(1):111-8. doi: 10.1111/j.1574-6968.1998.tb12899.x.
5
The L1 major capsid protein of human papillomavirus type 16 variants affects yield of virus-like particles produced in an insect cell expression system.人乳头瘤病毒16型变体的L1主要衣壳蛋白影响昆虫细胞表达系统中产生的病毒样颗粒的产量。
J Clin Microbiol. 1998 Jul;36(7):2046-51. doi: 10.1128/JCM.36.7.2046-2051.1998.
6
HPV antibody detection by ELISA with capsid protein L1 fused to glutathione S-transferase.采用与谷胱甘肽S-转移酶融合的衣壳蛋白L1，通过酶联免疫吸附测定法检测人乳头瘤病毒抗体。
J Virol Methods. 2002 Oct;106(1):61-70. doi: 10.1016/s0166-0934(02)00134-9.
7
Detection of antibodies to L1, L2, and E4 proteins of human papillomavirus types 6, 11, and 16 by ELISA using synthetic peptides.使用合成肽通过酶联免疫吸附测定法检测人乳头瘤病毒6型、11型和16型的L1、L2和E4蛋白抗体。
J Med Virol. 1995 Apr;45(4):410-4. doi: 10.1002/jmv.1890450410.
8
Antibodies against early proteins of human papillomaviruses as diagnostic markers for invasive cervical cancer.抗人乳头瘤病毒早期蛋白的抗体作为浸润性宫颈癌的诊断标志物
J Clin Microbiol. 1998 Feb;36(2):475-80. doi: 10.1128/JCM.36.2.475-480.1998.
9
Nasal immunization of mice with peptide having a cross-neutralization epitope on minor capsid protein L2 of human papillomavirus type 16 elicit systemic and mucosal antibodies.用具有人乳头瘤病毒16型次要衣壳蛋白L2上交叉中和表位的肽对小鼠进行鼻腔免疫可引发全身和粘膜抗体。
Vaccine. 2001 Jan 8;19(11-12):1496-502. doi: 10.1016/s0264-410x(00)00367-4.
10
Expression of the full-length products of the human papillomavirus type 6b (HPV-6b) and HPV-11 L2 open reading frames by recombinant baculovirus, and antigenic comparisons with HPV-11 whole virus particles.
J Gen Virol. 1990 Nov;71 ( Pt 11):2725-9. doi: 10.1099/0022-1317-71-11-2725.
引用本文的文献
1
Current and future direction in treatment of HPV-related cervical disease.当前和未来治疗 HPV 相关宫颈疾病的方向。
J Mol Med (Berl). 2022 Jun;100(6):829-845. doi: 10.1007/s00109-022-02199-y. Epub 2022 Apr 27.
2
Role of human papillomavirus types 16, 18, and 52 in recurrent cystitis and urinary bladder cancer among Egyptian patients.人乳头瘤病毒16型、18型和52型在埃及患者复发性膀胱炎和膀胱癌中的作用。
Medscape J Med. 2008;10(10):232. Epub 2008 Oct 8.
3
Determinants of human papillomavirus 16 serological conversion and persistence in a population-based cohort of 10 000 women in Costa Rica.哥斯达黎加10000名女性人群中，人乳头瘤病毒16型血清学转换和持续感染的决定因素
Br J Cancer. 2004 Oct 4;91(7):1269-74. doi: 10.1038/sj.bjc.6602088.
4
Seroprevalence of human papillomavirus-16, -18, -31, and -45 in a population-based cohort of 10000 women in Costa Rica.哥斯达黎加一个基于人群的10000名女性队列中人乳头瘤病毒16、18、31和45型的血清流行率。
Br J Cancer. 2003 Oct 6;89(7):1248-54. doi: 10.1038/sj.bjc.6601272.
5
The epidemiology of conjunctival squamous cell carcinoma in Uganda.乌干达结膜鳞状细胞癌的流行病学
Br J Cancer. 2002 Jul 29;87(3):301-8. doi: 10.1038/sj.bjc.6600451.
6
Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus.用于检测人乳头瘤病毒病毒样颗粒抗体的增强酶联免疫吸附测定法。
J Clin Microbiol. 2002 May;40(5):1755-60. doi: 10.1128/JCM.40.5.1755-1760.2002.
7
Prevalence of anti-human papillomavirus type 16, 18, 31, and 58 virus-like particles in women in the general population and in prostitutes.普通人群及性工作者中抗人乳头瘤病毒16型、18型、31型和58型病毒样颗粒的流行情况
J Clin Microbiol. 2001 Dec;39(12):4344-8. doi: 10.1128/JCM.39.12.4344-4348.2001.
8
In vitro gene transfer using human papillomavirus-like particles.使用人乳头瘤病毒样颗粒进行体外基因转移。
Nucleic Acids Res. 1998 Mar 1;26(5):1317-23. doi: 10.1093/nar/26.5.1317.
9
Immunomanipulative strategies for the control of human papillomavirus associated cervical disease.用于控制人乳头瘤病毒相关宫颈疾病的免疫调控策略
Immunol Res. 1997;16(4):387-400. doi: 10.1007/BF02786401.
10
Antibodies to human papillomavirus 16, 18, 58, and 6b major capsid proteins among Japanese females.日本女性中针对人乳头瘤病毒16、18、58和6b型主要衣壳蛋白的抗体
Jpn J Cancer Res. 1997 Apr;88(4):369-75. doi: 10.1111/j.1349-7006.1997.tb00391.x.
本文引用的文献
1
Evolution of the antibody response to human papillomavirus type 11 (HPV-11) in patients with condyloma acuminatum according to treatment response.根据治疗反应，尖锐湿疣患者对11型人乳头瘤病毒（HPV-11）抗体反应的演变
J Med Virol. 1993 Apr;39(4):340-4. doi: 10.1002/jmv.1890390414.
2
Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles.人乳头瘤病毒11型L1蛋白在昆虫细胞中的表达：病毒样颗粒的体内和体外组装
J Virol. 1993 Apr;67(4):1936-44. doi: 10.1128/JVI.67.4.1936-1944.1993.
3
Self-assembly of human papillomavirus type 16 capsids by expression of the L1 protein in insect cells.通过在昆虫细胞中表达L1蛋白实现人乳头瘤病毒16型衣壳的自组装。
FEMS Microbiol Lett. 1994 Apr 15;117(3):269-74. doi: 10.1111/j.1574-6968.1994.tb06778.x.
4
A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16.一种病毒样颗粒酶联免疫吸附测定法可检测出大多数感染人乳头瘤病毒16型的女性血清中的抗体。
J Natl Cancer Inst. 1994 Apr 6;86(7):494-9. doi: 10.1093/jnci/86.7.494.
5
HPV-1 capsids expressed in vitro detect human serum antibodies associated with foot warts.在体外表达的HPV-1衣壳可检测出与足部疣相关的人血清抗体。
Virology. 1993 Aug;195(2):456-62. doi: 10.1006/viro.1993.1396.
6
Role of conformational epitopes expressed by human papillomavirus major capsid proteins in the serologic detection of infection and prophylactic vaccination.
Gynecol Oncol. 1994 Oct;55(1):13-20. doi: 10.1006/gyno.1994.1239.
7
Monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11.单克隆抗体介导的对人乳头瘤病毒11型感染性的中和作用
J Virol. 1990 Nov;64(11):5678-81. doi: 10.1128/JVI.64.11.5678-5681.1990.
8
Prevalence of antibodies to human papillomavirus type 8 in human sera.人血清中抗8型人乳头瘤病毒抗体的流行率
J Virol. 1990 Sep;64(9):4399-406. doi: 10.1128/JVI.64.9.4399-4406.1990.
9
Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus.利用重组痘苗病毒制备抗人乳头瘤病毒16型单克隆抗体及其特性鉴定
J Clin Pathol. 1990 Jun;43(6):488-92. doi: 10.1136/jcp.43.6.488.
10
Evidence of prevalent genital-type human papillomavirus infections in adults and children.成人和儿童中普遍存在生殖器型人乳头瘤病毒感染的证据。
J Infect Dis. 1990 Jul;162(1):60-9. doi: 10.1093/infdis/162.1.60.
Zotero 插件