Müller-Richter Urs D A, Dowejko Albert, Zhou Weiqi, Reichert Torsten E, Driemel Oliver
Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, Germany.
Oral Oncol. 2008 Jul;44(7):628-33. doi: 10.1016/j.oraloncology.2007.08.007. Epub 2007 Nov 13.
MAGE-A-antigens are an immunologic marker for many cancers. The goal of this study was to compare the expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in foetal and adult keratinocytes with an oral squamous cell carcinoma (OSCC) cell line. Expression of MAGE-A2, -A3, -A4, -A6 and -A10-antigens were detected with PCR in foetal and adult keratinocyte cell lines and in an OSCC cell line (pT4N1M0). Quantitative expression of the single MAGE-A-antigens was measured with rtq-PCR. The results were compared to the reference value of the adult keratinocyte cell line. MAGE-A-antigens were detected in all cell lines. Expression profiles of adult and foetal keratinocyte cell lines differed significantly. Expression profiles of foetal and carcinoma cell lines differed significantly also. MAGE-A-antigens were detected in foetal keratinocyte cell line and oral squamous cell carcinoma cell line but differ in their expression profiles. Up to now MAGE-A-antigens were not detected in foetal keratinocytes. Their role is still unknown.
黑色素瘤相关抗原A（MAGE-A）是多种癌症的免疫标志物。本研究的目的是比较胎儿和成人角质形成细胞与口腔鳞状细胞癌（OSCC）细胞系中MAGE-A2、-A3、-A4、-A6和-A10的表达谱。采用聚合酶链反应（PCR）检测胎儿和成人角质形成细胞系以及一个OSCC细胞系（pT4N1M0）中MAGE-A2、-A3、-A4、-A6和-A10抗原的表达。用逆转录定量聚合酶链反应（rtq-PCR）测定单个MAGE-A抗原的定量表达。将结果与成人角质形成细胞系的参考值进行比较。在所有细胞系中均检测到MAGE-A抗原。成人和胎儿角质形成细胞系的表达谱有显著差异。胎儿和癌细胞系的表达谱也有显著差异。在胎儿角质形成细胞系和口腔鳞状细胞癌细胞系中检测到MAGE-A抗原，但它们的表达谱不同。到目前为止，在胎儿角质形成细胞中未检测到MAGE-A抗原。它们的作用仍然未知。
