Piotrowski J, Slomiany A, Murty V L, Fekete Z, Slomiany B L
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Biochem Int. 1991 Jul;24(4):749-56.
The role of human gastric mucin in mucosal protection against Helicobacter pylori colonization was investigated. H. pylori cells were incubated with purified intact mucin or its acidic fractions and then examined for their inhibitory capacity of H. pylori attachment to erythrocytes. Titration data established that the inhibitory activity of mucin was associated with its acidic component as the fraction enriched in sialic acid and sulfate showed 16-fold higher inhibitory titer than that of the intact mucin. While the inhibitory titer of acidic mucin fraction was not affected by the removal of sialic acid, the desulfation led to a complete loss of its inhibitory activity, thus pointing towards the importance of sulfate ester groups in this process. The results for the first time point towards the involvement of sulfomucins in the protection of gastric mucosa against colonization by H. pylori.
研究了人胃黏液在黏膜抵御幽门螺杆菌定植中的作用。将幽门螺杆菌细胞与纯化的完整黏液或其酸性组分一起孵育，然后检测它们对幽门螺杆菌黏附红细胞的抑制能力。滴定数据表明，黏液的抑制活性与其酸性成分有关，因为富含唾液酸和硫酸盐的组分显示出比完整黏液高16倍的抑制效价。虽然酸性黏液组分的抑制效价不受唾液酸去除的影响，但脱硫导致其抑制活性完全丧失，从而表明硫酸酯基团在此过程中的重要性。这些结果首次表明硫酸黏蛋白参与胃黏膜抵御幽门螺杆菌定植的过程。
