Krasheninnikov I A, Komar A A, Adzhubei I A
Department of Molecular Biology, Faculty of Biology, Moscow State University, USSR.
J Protein Chem. 1991 Oct;10(5):445-53. doi: 10.1007/BF01025472.
Examination of nascent globin peptides accumulating in vitro during globin synthesis in rabbit reticulocyte lysates was carried out. A view was supported that nonrandom distribution of codons with different usage frequencies in mRNA may determine the messenger's translation kinetics. Regions of reduced translation of alpha- and beta-globin polypeptide chains were localized, and the cotranslational protein-folding model suggested previously was substantiated. An active conjunction of synthesis and folding of proteins was proposed as one of the main destinations of a translation nonuniformity.
对兔网织红细胞裂解物中珠蛋白合成过程中体外积累的新生珠蛋白肽进行了检测。一种观点得到了支持，即mRNA中具有不同使用频率的密码子的非随机分布可能决定信使的翻译动力学。确定了α-和β-珠蛋白多肽链翻译减少的区域，并且先前提出的共翻译蛋白质折叠模型得到了证实。蛋白质合成与折叠的活跃结合被认为是翻译不均一性的主要目的之一。
