Ma Chao-Mei, Hattori Masao, Chen Hu-Biao, Cai Shao-Qing, Daneshtalab Mohsen
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Phytochem Anal. 2008 Jul-Aug;19(4):294-300. doi: 10.1002/pca.1045.
An HPLC-PAD-MS(n) method was employed to profile the phenolic compounds of the aerial part of Artemisia pectinata (Neopallasia pectinata), a plant with no previous reports concerning its phenolic constituents. Three isomers of trans-caffeoylquinic acid accompanied by cis-5-caffeoylquinic acid, six isomers of trans-dicaffeoylquinic acid, two isomers of methyl trans-dicaffeoylquinate (including one new isomer), a trans-caffeoylferuloylquinic acid and three flavanoids were identified unambiguously by analysis of their UV and MS(n) spectra in comparison with standard compounds that were isolated from natural sources, or synthesised, or were surrogate standards (green coffee extract). Other compounds were identified by analysis of their UV and MSn data in comparison with those reported in the literature. MS(n) experiments also suggested the presence of groups of dicaffeoylquinic acid glycosides, caffeoylquinic acid diglycosides, caffeoylquinic acid glycosides and quinic acid diglycosides.
采用高效液相色谱-光电二极管阵列-质谱(n)法对栉叶蒿（新栉叶蒿）地上部分的酚类化合物进行分析，此前尚无关于该植物酚类成分的报道。通过与从天然来源分离、合成或作为替代标准品（绿咖啡提取物）的标准化合物比较其紫外光谱和质谱(n)光谱，明确鉴定出三种反式咖啡酰奎宁酸异构体以及顺式-5-咖啡酰奎宁酸、六种反式二咖啡酰奎宁酸异构体、两种反式二咖啡酰奎宁酸甲酯异构体（包括一种新异构体）、一种反式咖啡酰阿魏酰奎宁酸和三种黄酮类化合物。通过与文献报道的数据比较其紫外光谱和质谱(n)数据，鉴定出其他化合物。质谱(n)实验还表明存在二咖啡酰奎宁酸糖苷、咖啡酰奎宁酸二糖苷、咖啡酰奎宁酸糖苷和奎宁酸二糖苷组。
