Centro de Química da Madeira, Departamento de Química, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Portugal.
Rapid Commun Mass Spectrom. 2009 Dec;23(24):3939-53. doi: 10.1002/rcm.4335.
A simple and rapid method has been used for the screening and identification of the main phenolic compounds from Helichrysum devium using high-performance liquid chromatography with on-line UV and electrospray ionization mass spectrometric detection (LC-DAD/ESI-MS(n)). The total aerial parts and different morphological parts of the plant, namely leaves, flowers and stems, were analyzed separately. A total of 34 compounds present in the methanolic extract from Helichrysum devium were identified or tentatively characterized based on their UV and mass spectra and retention times. Three of these compounds were positively identified by comparison with reference standards. The phenolic compounds included derivatives of quinic acid, O-glycosylated flavonoids, a caffeic acid derivative and a protocatechuic acid derivative. The characteristic loss of 206 Da from malonylcaffeoyl quinic acid was used to confirm the malonyl linkage to the caffeoyl group. This contribution presents one of the first reports on the analysis of phenolic compounds from Helichrysum devium using LC-DAD/ESI-MS(n) and highlights the prominence of quinic acid derivatives as the main group of phenolic compounds present in these extracts. We also provide evidence that the methanolic extract from the flowers was significantly more complex when compared to that of other morphological parts.
已采用高效液相色谱-在线紫外和电喷雾电离质谱联用（LC-DAD/ESI-MS(n)）法对蜡菊中的主要酚类化合物进行筛选和鉴定，该方法简单快捷。对植物的地上部分和不同形态部分（即叶、花和茎）的总部分和不同形态部分分别进行了分析。根据 UV 和质谱以及保留时间，从蜡菊的甲醇提取物中鉴定或暂定鉴定了 34 种化合物。其中 3 种化合物通过与对照品比较进行了阳性鉴定。这些酚类化合物包括奎尼酸衍生物、O-糖基化黄酮类、咖啡酸衍生物和原儿茶酸衍生物。从丙二酰咖啡酰奎宁酸中特征性地丢失 206 Da 用于确认丙二酰基与咖啡酰基的连接。本研究首次采用 LC-DAD/ESI-MS(n)分析蜡菊中的酚类化合物，并强调了奎尼酸衍生物作为这些提取物中主要酚类化合物的显著地位。我们还提供了证据表明，与其他形态部分相比，花的甲醇提取物明显更复杂。
