Kishida A, Iwata H, Tamada Y, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1991 Oct;12(8):786-92. doi: 10.1016/0142-9612(91)90031-5.
Following exposure to corona discharge, a polyethylene film was graft polymerized with different water-soluble monomers such as acrylamide (non-ionic), acrylic acid (anionic), 2-acrylamide-2-methyl propane sulphonic acid (anionic), styrene sulphonic acid sodium salt (anionic) and N,N-dimethylaminopropyl acrylamide (cationic). Attachment and proliferation of HeLa S3 cells were studied for grafted surfaces with different zeta potentials and contact angles. The polyethylene surface graft polymerized with styrene sulphonic acid sodium salt exhibited high cell attachment and protein adsorption, whereas the cells did not adhere to the 2-acrylamide-2-methyl propane sulphonic acid graft-polymerized surface, although both surfaces had high negative zeta potentials. Graft polymerization of acrylamide reduced the zeta potential of surface close to zero and rejected the cell attachment. The polyethylene surface became highly cell-adhesive through graft polymerization of the cationic N,N-dimethylaminopropyl acrylamide monomer, but too much grafting killed the attaching cells. Once the cells attached to a surface without being killed, they could proliferate at the same growth rate, whatever their surface zeta potential.
在暴露于电晕放电后，将聚乙烯薄膜与不同的水溶性单体进行接枝聚合，这些单体包括丙烯酰胺（非离子型）、丙烯酸（阴离子型）、2-丙烯酰胺-2-甲基丙烷磺酸（阴离子型）、苯乙烯磺酸钠盐（阴离子型）和N,N-二甲基氨基丙基丙烯酰胺（阳离子型）。研究了具有不同zeta电位和接触角的接枝表面上HeLa S3细胞的附着和增殖情况。用苯乙烯磺酸钠盐接枝聚合的聚乙烯表面表现出高细胞附着和蛋白质吸附，而细胞不粘附于2-丙烯酰胺-2-甲基丙烷磺酸接枝聚合的表面，尽管两个表面都具有高负zeta电位。丙烯酰胺的接枝聚合使表面的zeta电位降低至接近零，并抑制了细胞附着。通过阳离子N,N-二甲基氨基丙基丙烯酰胺单体的接枝聚合，聚乙烯表面变得具有高度细胞粘附性，但接枝过多会杀死附着的细胞。一旦细胞附着在未被杀死的表面上，无论其表面zeta电位如何，它们都能以相同的生长速率增殖。
