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阳离子改性磷酸胆碱聚合物对人成骨细胞的体外作用及其对体内骨形成的影响。

The effect of cationically-modified phosphorylcholine polymers on human osteoblasts in vitro and their effect on bone formation in vivo.

机构信息

Department of Materials Science and Metallurgy, Cambridge Centre for Medical Materials, University of Cambridge, New Museum Site, Cambridge, CB2 3QZ, UK.

Orthopaedic Research Unit, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge, CB2 2QQ, UK.

出版信息

J Mater Sci Mater Med. 2017 Aug 17;28(9):144. doi: 10.1007/s10856-017-5958-8.

DOI:10.1007/s10856-017-5958-8
PMID:28819908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5561156/
Abstract

The effect of introducing cationic charge into phosphorylcholine (PC)-based polymers has been investigated in this study with a view to using these materials as coatings to improve bone formation and osseointegration at the bone-implant interface. PC-based polymers, which have been used in a variety of medical devices to improve biocompatibility, are associated with low protein adsorption resulting in reduced complement activation, inflammatory response and cell adhesion. However, in some applications, such as orthopaedics, good integration between the implant and bone is needed to allow the distribution of loading stresses and a bioactive response is required. It has previously been shown that the incorporation of cationic charge into PC-based polymers may increase protein adsorption that stimulates subsequent cell adhesion. In this paper, the effect of cationic charge in PC-based polymers on human osteoblasts (HObs) in vitro and the effect of these polymers on bone formation in the rat tibia was assessed. Increasing PC positive surface charge increased HOb cell adhesion and stimulated increased cell differentiation and the production of calcium phosphate deposits. However, when implanted in bone these materials were at best biotolerant, stimulating the production of fibrous tissue and areas of loosely associated matrix (LAM) around the implant. Their development, as formulated in this study, as bone interfacing implant coatings is therefore not warranted.

摘要

本研究考察了在磷酸胆碱（PC）基聚合物中引入正电荷对其的影响，以期将这些材料用作涂层，以改善骨-植入物界面的骨形成和骨整合。PC 基聚合物已被用于多种医疗器械中以提高生物相容性，但其与低蛋白吸附相关，导致补体激活、炎症反应和细胞黏附减少。然而，在一些应用中，如矫形外科，需要植入物与骨骼之间有良好的整合，以允许分布加载应力，并需要生物活性反应。先前已经表明，在 PC 基聚合物中引入正电荷可以增加刺激随后细胞黏附的蛋白质吸附。在本文中，评估了 PC 基聚合物中正电荷对人成骨细胞（HObs）的体外影响，以及这些聚合物对大鼠胫骨骨形成的影响。增加 PC 正表面电荷可增加 HOb 细胞黏附，并刺激细胞分化和钙磷沉积物的产生。然而，当将这些材料植入骨中时，它们充其量是生物耐受的，会刺激纤维组织和植入物周围松散结合基质（LAM）区域的产生。因此，就本研究中提出的观点而言，它们不适合作为骨界面植入物涂层。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/27f214541aa8/10856_2017_5958_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/5c7d612c05a9/10856_2017_5958_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/166ca922b5c0/10856_2017_5958_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/50ec9e92d2be/10856_2017_5958_Fig4_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/e993ee9c5a62/10856_2017_5958_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/9cc9fea01b3e/10856_2017_5958_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/27f214541aa8/10856_2017_5958_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/5c7d612c05a9/10856_2017_5958_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/166ca922b5c0/10856_2017_5958_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/5f1110a85a17/10856_2017_5958_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/50ec9e92d2be/10856_2017_5958_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/4f7b5c90a6ec/10856_2017_5958_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/e993ee9c5a62/10856_2017_5958_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/9cc9fea01b3e/10856_2017_5958_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d5d/5561156/27f214541aa8/10856_2017_5958_Fig8_HTML.jpg

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