Lee J H, Jung H W, Kang I K, Lee H B
Department of Macromolecular Science, Han Nam University, Taejeon, Korea.
Biomaterials. 1994 Jul;15(9):705-11. doi: 10.1016/0142-9612(94)90169-4.
Surfaces with differently chargeable functional groups were prepared on low density polyethylene sheets by corona discharge treatment, followed by graft copolymerization of acrylic acid (-COOH, negatively chargeable) and a subsequent substitution reaction of carboxylic acid groups to hydroxyl (-CH2OH, neutral) or amide (-CONH2, neutral) groups. The amide groups grafted on the surface were further converted to amine groups (-CH2NH2, positively chargeable). The prepared surfaces were characterized by measuring the water contact angle, electron spectroscopy for chemical analysis and Fourier-transform infrared spectroscopy in the attenuated total reflectance mode. It was observed that the wettability of the different functional group-grafted surfaces largely increases compared with the control surface but is not much affected by the kind of functional groups grafted. The interaction of Chinese hamster ovary cells with the functional group-grafted surfaces was investigated. The cells adhered and grown on the surfaces were counted using an electronic cell counter and observed by a scanning electron microscope. The surface grafted with amine groups was best for cell adhesion, spreading and growth probably owing to the positively chargeable character in aqueous cell culture medium. For surfaces grafted with neutral functional groups, the surface grafted with hydroxyl groups showed better cell spreading than that grafted with amide groups.
通过电晕放电处理在低密度聚乙烯片材上制备具有不同可充电官能团的表面，随后进行丙烯酸（-COOH，可带负电荷）的接枝共聚，以及羧酸基团向羟基（-CH2OH，中性）或酰胺（-CONH2，中性）基团的后续取代反应。接枝在表面上的酰胺基团进一步转化为胺基团（-CH2NH2，可带正电荷）。通过测量水接触角、化学分析电子能谱和衰减全反射模式下的傅里叶变换红外光谱对制备的表面进行表征。观察到与对照表面相比，不同官能团接枝表面的润湿性大大增加，但受接枝官能团种类的影响不大。研究了中国仓鼠卵巢细胞与官能团接枝表面的相互作用。使用电子细胞计数器对粘附并生长在表面上的细胞进行计数，并通过扫描电子显微镜进行观察。接枝有胺基团的表面最有利于细胞粘附、铺展和生长，这可能是由于在水性细胞培养基中具有可带正电荷的特性。对于接枝有中性官能团的表面，接枝有羟基的表面比接枝有酰胺基团的表面表现出更好的细胞铺展。
