Pinto Sofia B, Kafatos Fotis C, Michel Kristin
Imperial College London, Faculty of Natural Sciences, Division of Cell and Molecular Biology, Sir Alexander Fleming Building, South Kensington Campus, London, SW7 2AZ, UK.
Cell Microbiol. 2008 Apr;10(4):891-8. doi: 10.1111/j.1462-5822.2007.01091.x. Epub 2007 Nov 14.
For malaria transmission to occur, Plasmodium sporozoites must infect the salivary glands of their mosquito vectors. This study reports that Anopheles gambiae SRPN6 participates in a local salivary gland epithelial response against the rodent malaria parasite, Plasmodium berghei. We showed previously that SRPN6, an immune inducible midgut invasion marker, influences ookinete development. Here we report that SRPN6 is also specifically induced in salivary glands with the onset of sporozoite invasion. The protein is located in the basal region of epithelial cells in proximity to invading sporozoites. Knockdown of SRPN6 during the late phase of sporogony by RNAi has no effect on oocyst rupture but significantly increases the number of sporozoites present in salivary glands. Despite several differences between the passage of Plasmodium through the midgut and the salivary glands, this study identifies a striking overlap in the molecular responses of these two epithelia to parasite invasion.
为了实现疟疾传播，疟原虫子孢子必须感染其蚊媒的唾液腺。本研究报告称，冈比亚按蚊SRPN6参与了唾液腺上皮对啮齿动物疟原虫伯氏疟原虫的局部反应。我们之前表明，SRPN6是一种免疫诱导的中肠入侵标志物，会影响动合子的发育。在此我们报告，随着子孢子入侵的开始，SRPN6在唾液腺中也被特异性诱导。该蛋白位于上皮细胞的基部区域，靠近入侵的子孢子。在孢子生殖后期通过RNA干扰敲低SRPN6对卵囊破裂没有影响，但显著增加了唾液腺中存在的子孢子数量。尽管疟原虫通过中肠和唾液腺的过程存在一些差异，但本研究确定了这两种上皮对寄生虫入侵的分子反应存在显著重叠。
