Choumet Valérie, Carmi-Leroy Annick, Laurent Christine, Lenormand Pascal, Rousselle Jean-Claude, Namane Abdelkader, Roth Charles, Brey Paul T
Unité de Biochimie et de Biologie Moléculaire des Insectes, Institut Pasteur, Paris cedex 15, France.
Proteomics. 2007 Sep;7(18):3384-94. doi: 10.1002/pmic.200700334.
Proteins synthesized in the salivary glands of the Anopheles gambiae mosquito are thought to be important in the life cycle of the malaria parasite Plasmodium. To describe A. gambiae salivary gland and saliva contents, we combined several techniques: 1-DE, 2-DE and LC MS/MS. This study has identified five saliva proteins and 122 more proteins from the salivary glands, including the first proteomic description for 89 of these salivary gland proteins. Since the invasion and sporozoite maturation take place during the process of salivary glands ageing, the effect of salivary gland age on salivary component composition was examined. LC MS/MS profiling of young versus old salivary gland proteomes suggests that there is an over-representation of proteins involved in signaling and proteins related to the immune response in the proteins from older mosquitoes. The iTRAQ labeling was used for a comparative proteomic analysis of salivary gland samples from infected or Plasmodium berghei-free mosquitoes. The expression levels of five secreted proteins were altered when the parasite was present. These observations will serve as a basis for future work concerning the possible role of these proteins in the interaction between A. gambiae, Plasmodium and the mammalian host.
冈比亚按蚊唾液腺中合成的蛋白质被认为在疟原虫疟原虫的生命周期中很重要。为了描述冈比亚按蚊唾液腺和唾液成分，我们结合了几种技术：一维电泳、二维电泳和液相色谱-串联质谱。这项研究鉴定出了五种唾液蛋白和唾液腺中的另外122种蛋白质，其中包括对89种唾液腺蛋白的首次蛋白质组学描述。由于入侵和子孢子成熟发生在唾液腺老化过程中，因此研究了唾液腺年龄对唾液成分组成的影响。年轻与老年唾液腺蛋白质组的液相色谱-串联质谱分析表明，老年蚊子的蛋白质中参与信号传导的蛋白质和与免疫反应相关的蛋白质比例过高。iTRAQ标记用于对感染或未感染伯氏疟原虫的蚊子的唾液腺样本进行比较蛋白质组分析。当寄生虫存在时，五种分泌蛋白的表达水平发生了改变。这些观察结果将为今后研究这些蛋白质在冈比亚按蚊、疟原虫和哺乳动物宿主之间相互作用中可能发挥的作用奠定基础。
