Masuda Shinako, Shimizu Tatsuya, Yamato Masayuki, Okano Teruo
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan.
Adv Drug Deliv Rev. 2008 Jan 14;60(2):277-85. doi: 10.1016/j.addr.2007.08.031. Epub 2007 Oct 9.
Recently, myocardial tissue engineering has emerged as one of the most promising therapies for patients suffering from severe heart failure. Nevertheless, conventional methods in tissue engineering involving the seeding of cells into biodegradable scaffolds have intrinsic shortcomings, such as inflammatory reactions and fibrous tissue formation caused by scaffold degradation. On the other hand, we have developed cell sheet engineering as scaffoldless tissue engineering, and applied it for myocardial tissue engineering. Using temperature-responsive culture surfaces, cells can be harvested as intact sheets and cell-dense thick tissues are constructed by layering these cell sheets. Myocardial cell sheets non-invasively harvested from temperature-responsive culture surfaces are successfully layered, resulting in electrically communicative 3-dimensional (3-D) cardiac constructs. Transplantation of cell sheets onto damaged hearts improved heart function in several animal models. In this review, we summarize the development of myocardial tissue engineering using cell sheets harvested from temperature-responsive culture surfaces and discuss about future views.
最近，心肌组织工程已成为治疗严重心力衰竭患者最有前景的疗法之一。然而，组织工程中的传统方法，即将细胞接种到可生物降解支架中，存在内在缺陷，如支架降解引起的炎症反应和纤维组织形成。另一方面，我们已开发出细胞片工程作为无支架组织工程，并将其应用于心肌组织工程。利用温度响应性培养表面，细胞可以完整的薄片形式收获，通过堆叠这些细胞片构建细胞密集的厚组织。从温度响应性培养表面无创收获的心肌细胞片成功堆叠，形成具有电传导性的三维（3-D）心脏构建体。在几种动物模型中，将细胞片移植到受损心脏上可改善心脏功能。在本综述中，我们总结了利用从温度响应性培养表面收获的细胞片进行心肌组织工程的进展，并探讨其未来前景。
