Vaidya Sunil R, Wairagkar Niteen S, Raja D, Khedekar Deepika D, Gunasekaran P, Shankar S, Mahadevan Anita, Ramamurty Nalini
Measles Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, PO Box 11, Pune 411001, India.
Virus Genes. 2008 Feb;36(1):31-4. doi: 10.1007/s11262-007-0172-2. Epub 2007 Nov 16.
National Institute of Virology, India has instituted molecular surveillance of measles strains in the country. In phased manner, three major cities Pune, Chennai and Bangalore were covered. Throat swab and urine from suspected measles cases from Chennai and Pune and freshly frozen brain tissues, CSF from suspected SSPE case from Bangalore were subjected to RNA extraction and Measles N&H gene RT-PCR as per WHO standard protocols. PCR positive products were sequenced. Sequence alignment and phylogenetic analysis was carried out using WHO standard reference sequences. Virus isolation was attempted using B95a cell line. Measles genotype D7 was detected from two classical measles cases (Chennai and Pune) and in a fulminant SSPE case (Bangalore). This is the first detection of measles genotype D7 from India.
印度国家病毒研究所已在该国开展麻疹毒株的分子监测。分阶段覆盖了三个主要城市浦那、金奈和班加罗尔。按照世界卫生组织的标准方案，对来自金奈和浦那的疑似麻疹病例的咽拭子和尿液，以及来自班加罗尔疑似亚急性硬化性全脑炎（SSPE）病例的新鲜冷冻脑组织、脑脊液进行RNA提取和麻疹N基因与H基因逆转录聚合酶链反应（RT-PCR）。对PCR阳性产物进行测序。使用世界卫生组织标准参考序列进行序列比对和系统发育分析。尝试使用B95a细胞系进行病毒分离。在两例典型麻疹病例（金奈和浦那）以及一例暴发性SSPE病例（班加罗尔）中检测到麻疹基因型D7。这是在印度首次检测到麻疹基因型D7。
