Rogan M T, Craig P S, Zeyhle E, Romig T, Lubano G M, Deshan L
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, UK.
Trans R Soc Trop Med Hyg. 1991 Nov-Dec;85(6):773-7. doi: 10.1016/0035-9203(91)90451-4.
A rapid dot enzyme-linked immunosorbent assay (dot-ELISA) was developed as a field test for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The 30 min test was based on the detection of antibodies to antigen B of hydatid fluid and was carried out using 50 microliters of whole blood in a field assessment in the Turkana region of north-west Kenya. Initial laboratory studies showed antigen B to be preferable to crude cyst fluid, with 94% sensitivity and 90.3% specificity for Echinococcus infections. The field test was rapid, inexpensive and simple to perform and is considered to be a useful back-up to ultrasound scanning.
一种快速斑点酶联免疫吸附测定法（斑点酶联免疫吸附测定）被开发出来作为诊断由细粒棘球绦虫引起的囊型包虫病的现场检测方法。这项30分钟的检测基于对包虫囊液抗原B抗体的检测，并且在肯尼亚西北部图尔卡纳地区的现场评估中使用50微升全血进行。初步实验室研究表明，抗原B比粗制囊液更具优势，对棘球绦虫感染的敏感性为94%，特异性为90.3%。该现场检测快速、廉价且操作简单，被认为是超声扫描的一种有用的辅助手段。
