Viganò P, Fusi F M, Brigante C, Busacca M, Vignali M
III Dept. of Obstetrics and Gynecology, University of Milano, Italy.
Andrologia. 1991 Sep-Oct;23(5):367-71. doi: 10.1111/j.1439-0272.1991.tb02582.x.
A method is described where superparamagnetic polymer microspheres coated with monoclonal antibodies are used to isolate antibody-labelled from antibody-free spermatozoa in male autoimmune infertility. Autoimmune sperm samples or antibody-free spermatozoa adsorbed with antisperm-antibodies from sera were incubated with microspheres coated with a specific monoclonal antibody to murine immunoglobulins, after their preincubation with mouse anti-human IgG and IgA. Using a magnet, the microsphere-labelled spermatozoa were separated from the samples. Immunobead binding was performed before and after the treatment in order to detect changes in the percentage of antibody-bound spermatozoa. After the immunomagnetic separation, approximately 50% of the IgA-labelled spermatozoa was isolated while no difference was demonstrated when antisperm antibodies of IgG class were involved. The evaluation of sperm motility and membrane integrity after treatment seemed to indicate that the technique did not have any relevant effect on sperm characteristics. The fact that only a partial success in separation of IgA-bound spermatozoa and no success for IgG-labelled sperm was obtained indicates that the method needs to be improved before its clinical utilization might be postulated.
本文描述了一种方法，即在男性自身免疫性不育症中，使用包被有单克隆抗体的超顺磁性聚合物微球从无抗体的精子中分离出抗体标记的精子。自身免疫性精子样本或用血清中的抗精子抗体吸附的无抗体精子，在与小鼠抗人IgG和IgA预孵育后，与包被有针对鼠免疫球蛋白的特异性单克隆抗体的微球一起孵育。使用磁铁将微球标记的精子从样本中分离出来。在处理前后进行免疫珠结合检测，以检测抗体结合精子百分比的变化。免疫磁分离后，约50%的IgA标记精子被分离出来，而当涉及IgG类抗精子抗体时未显示出差异。处理后对精子活力和膜完整性的评估似乎表明该技术对精子特性没有任何相关影响。仅在分离IgA结合精子方面取得部分成功，而对IgG标记精子未取得成功，这一事实表明该方法在可假定其临床应用之前需要改进。
