Kiser G C, Alexander N J, Fuchs E F, Fulgham D L
Fertil Steril. 1987 Mar;47(3):466-74. doi: 10.1016/s0015-0282(16)59057-8.
Fourteen men with a mean duration of infertility greater than 3 years who had significant sperm immobilizing or sperm-agglutinating antibodies were studied. All patients had greater than 20% IgG or IgA immunobinding to sperm in their seminal plasma and 7 had immunobinding levels of greater than 50%. Sperm from these men were less able to penetrate an overlaying buffer layer than sperm from a fertile control. Addition of immunobeads to the specimen was of little use, because few motile sperm could swim into the overlaying buffer; retained immunobeads were noted in the buffer layer of 18-hour capacitated specimens. Magnetic isolation of antibody-coated sperm from antibody-free sperm avoids potential damage to fragile sperm through centrifugation. Viable spermatozoa were isolated from magnetite-complexed spermatozoa, but the motility of the isolated spermatozoa deteriorated rapidly during the subsequent capacitation period. Passage of diluted ejaculate through a column of dextran beads for antisperm antibody processing (ASAP) was associated with superior sperm quality and fertilizing potential. The use of ASAP resulted in good sperm velocity and linearity and improved sperm function, as measured with the hamster egg penetration test. Sperm from men with immunologically mediated infertility can be processed through the ASAP and used for artificial insemination of their partners or in an in vitro fertilization program.
对14名平均不孕时间超过3年且有显著精子制动或精子凝集抗体的男性进行了研究。所有患者精浆中IgG或IgA与精子的免疫结合率均超过20%，其中7名患者的免疫结合水平超过50%。与可育对照者的精子相比，这些男性的精子穿透上层缓冲液层的能力较弱。向标本中添加免疫珠用处不大，因为很少有活动精子能游入上层缓冲液；在18小时获能标本的缓冲液层中发现有残留的免疫珠。从无抗体精子中磁性分离抗体包被的精子可避免因离心对脆弱精子造成潜在损伤。从与磁铁矿复合的精子中分离出了活精子，但分离出的精子在随后的获能期运动能力迅速下降。将稀释的精液通过葡聚糖珠柱进行抗精子抗体处理（ASAP）可提高精子质量和受精潜力。使用ASAP可使精子速度和直线性良好，并改善精子功能，这通过仓鼠卵穿透试验来衡量。免疫介导性不孕男性的精子可通过ASAP处理，用于其伴侣的人工授精或体外受精程序。
