Hübner Yvonne, Hoettges Kai F, McDonnell Martin B, Carter Michael J, Hughes Michael P
Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey, UK.
Int J Nanomedicine. 2007;2(3):427-31.
A major problem for surface-based detection techniques such as surface plasmon resonance and quartz crystal microbalances is that at low concentrations, diffusion is an insufficient driving force to bring colloidal submicron-scale particles to the detection surface. In order to overcome this, it has previously been demonstrated that a combination of dielectrophoresis and AC-electro-hydrodynamic flow can be used to focus cell-sized particles from suspension onto a large metal surface, in order to improve the detection capabilities of such systems. In this paper we describe how the combination of these two phenomena, using the so-called "zipper" electrode array, can be used to concentrate a wide range of nanoparticles of biological interest, such as influenza virus, dissolved albumin, and DNA molecules as well as latex beads of various sizes. We also demonstrate that the speed at which particles are transported towards the centre of the electrode pads by dielectrophoresis and electro-hydrodynamic flow is not related to the particle size for colloidal particles.
诸如表面等离子体共振和石英晶体微天平这类基于表面的检测技术面临的一个主要问题是，在低浓度情况下，扩散作为驱动力不足以将胶体亚微米级颗粒带到检测表面。为了克服这一问题，此前已有研究表明，介电泳和交流电流体动力学流相结合可用于将悬浮液中细胞大小的颗粒聚焦到一个大金属表面上，以提高此类系统的检测能力。在本文中，我们描述了如何利用所谓的“拉链”电极阵列，通过这两种现象的结合来浓缩多种具有生物学意义的纳米颗粒，如流感病毒、溶解的白蛋白、DNA分子以及各种尺寸的乳胶珠。我们还证明，对于胶体颗粒而言，通过介电泳和电流体动力学流将颗粒向电极垫中心传输的速度与颗粒大小无关。
