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使用电生理和电化学方法测量嗜铬细胞中的分泌。

Measuring secretion in chromaffin cells using electrophysiological and electrochemical methods.

作者信息

Borges R, Camacho M, Gillis K D

机构信息

Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain.

出版信息

Acta Physiol (Oxf). 2008 Feb;192(2):173-84. doi: 10.1111/j.1748-1716.2007.01814.x. Epub 2007 Nov 16.

DOI:10.1111/j.1748-1716.2007.01814.x
PMID:18021323
Abstract

Our present understanding of exocytosis of catecholamines has benefited tremendously from the arrival of single-cell electrochemical methods (amperometry and voltammetry), electrophysiological techniques (whole-cell and patch capacitance) and from the combination of both techniques (patch amperometry). In this brief review, we will outline the strengths and limitations of amperometric and electrophysiological methods and highlight the major contribution obtained with the use of these techniques in chromaffin cells.

摘要

我们目前对儿茶酚胺胞吐作用的理解,极大地受益于单细胞电化学方法(安培法和伏安法)、电生理技术(全细胞和膜片电容)的出现,以及这两种技术的结合(膜片安培法)。在这篇简短的综述中,我们将概述安培法和电生理方法的优点和局限性,并强调在嗜铬细胞中使用这些技术所取得的主要贡献。

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