Physiologisches Institut, Universität des Saarlandes Homburg/Saar, Germany.
Front Neurosci. 2013 Nov 25;7:222. doi: 10.3389/fnins.2013.00222.
The last two decades have seen a tremendous development in high resolution microscopy techniques giving rise to acronyms such as TIRFM, SIM, PALM, STORM, and STED. The goal of all these techniques is to overcome the physical resolution barrier of light microscopy in order to resolve precise protein localization and possibly their interaction in cells. Neuroendocrine cell function is to secrete hormones and peptides on demand. This fine-tuned multi-step process is mediated by a large array of proteins. Here, we review the new microscopy techniques used to obtain high resolution and how they have been applied to increase our knowledge of the molecular mechanisms involved in neuroendocrine cell secretion. Further the limitations of these methods are discussed and insights in possible new applications are provided.
过去的二十年见证了高分辨率显微镜技术的飞速发展，出现了诸如 TIRFM、SIM、PALM、STORM 和 STED 等缩写词。所有这些技术的目标都是克服光显微镜的物理分辨率限制，以解析细胞中精确的蛋白质定位及其相互作用。神经内分泌细胞的功能是按需分泌激素和肽。这个精细的多步骤过程由大量蛋白质介导。在这里，我们综述了用于获得高分辨率的新显微镜技术，以及它们如何被应用于增加我们对神经内分泌细胞分泌相关分子机制的认识。进一步讨论了这些方法的局限性，并提供了对可能的新应用的见解。
