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本文引用的文献

1
Structural basis for transcription elongation by bacterial RNA polymerase.细菌RNA聚合酶转录延伸的结构基础。
Nature. 2007 Jul 12;448(7150):157-62. doi: 10.1038/nature05932. Epub 2007 Jun 20.
2
A novel lysozyme from Xanthomonas oryzae phage varphiXo411 active against Xanthomonas and Stenotrophomonas.一种来自水稻黄单胞菌噬菌体varphiXo411的新型溶菌酶，对黄单胞菌和嗜麦芽窄食单胞菌具有活性。
Protein Expr Purif. 2006 Dec;50(2):229-37. doi: 10.1016/j.pep.2006.06.013. Epub 2006 Jun 27.
3
Role of DNA bubble rewinding in enzymatic transcription termination.DNA气泡回绕在酶促转录终止中的作用。
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4870-5. doi: 10.1073/pnas.0600145103. Epub 2006 Mar 21.
4
The tale of two RNA polymerases: transcription profiling and gene expression strategy of bacteriophage Xp10.两种RNA聚合酶的故事：噬菌体Xp10的转录谱分析与基因表达策略
Mol Microbiol. 2005 Feb;55(3):764-77. doi: 10.1111/j.1365-2958.2004.04442.x.
5
A conserved zinc binding domain in the largest subunit of DNA-dependent RNA polymerase modulates intrinsic transcription termination and antitermination but does not stabilize the elongation complex.DNA依赖性RNA聚合酶最大亚基中一个保守的锌结合结构域可调节内在转录终止和抗终止，但不会稳定延伸复合物。
J Mol Biol. 2004 Sep 24;342(4):1143-54. doi: 10.1016/j.jmb.2004.07.072.
6
A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.一种通过靶向RNA聚合酶全酶的亚基间相互作用来抑制转录的调节因子。
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4554-9. doi: 10.1073/pnas.0400923101. Epub 2004 Mar 22.
7
A bacterial two-hybrid system based on transcription activation.一种基于转录激活的细菌双杂交系统。
Methods Mol Biol. 2004;261:231-46. doi: 10.1385/1-59259-762-9:231.
8
Bacteriophage-induced modifications of host RNA polymerase.噬菌体诱导的宿主RNA聚合酶修饰
Annu Rev Microbiol. 2003;57:301-22. doi: 10.1146/annurev.micro.57.030502.090942.
9
Genome of Xanthomonas oryzae bacteriophage Xp10: an odd T-odd phage.水稻白叶枯病菌噬菌体Xp10的基因组：一种奇特的T奇数噬菌体。
J Mol Biol. 2003 Jul 18;330(4):735-48. doi: 10.1016/s0022-2836(03)00634-x.
10
A novel bacteriophage-encoded RNA polymerase binding protein inhibits transcription initiation and abolishes transcription termination by host RNA polymerase.一种新型噬菌体编码的RNA聚合酶结合蛋白可抑制转录起始，并消除宿主RNA聚合酶的转录终止。
J Mol Biol. 2002 Jun 28;320(1):11-22. doi: 10.1016/S0022-2836(02)00420-5.

与噬菌体Xp10转录抗终止因子p7相互作用的RNA聚合酶残基的定位

Mapping of RNA polymerase residues that interact with bacteriophage Xp10 transcription antitermination factor p7.

作者信息

Yuzenkova Yulia, Zenkin Nikolay, Severinov Konstantin

机构信息

Waksman Institute for Microbiology Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.

出版信息

J Mol Biol. 2008 Jan 4;375(1):29-35. doi: 10.1016/j.jmb.2007.10.054. Epub 2007 Oct 25.

DOI:10.1016/j.jmb.2007.10.054
PMID:18021805
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2219388/
Abstract

Bacteriophage Xp10-encoded transcription factor p7 interacts with host Xanthomonas oryzae RNA polymerase beta' subunit and prevents both promoter recognition by the RNA polymerase holoenzyme and transcription termination by the RNA polymerase core. P7 does not bind to and has no effect on RNA polymerase from Escherichia coli. Here, we use a combination of biochemical and genetic methods to map the p7 interaction site to within four beta' amino acid residues at the N terminus of X. oryzae RNAP beta'. The interaction site is located in an area that is close to the promoter spacer in the open complex and to the upstream boundary of the transcription bubble in the elongation complex, providing a possible explanation of how a small protein can affect both transcription initiation and termination by binding to the same RNA polymerase site.

摘要

噬菌体Xp10编码的转录因子p7与宿主水稻黄单胞菌RNA聚合酶β'亚基相互作用，既能阻止RNA聚合酶全酶对启动子的识别，又能阻止RNA聚合酶核心酶的转录终止。P7不与大肠杆菌的RNA聚合酶结合，对其也没有影响。在这里，我们结合生化和遗传方法，将p7相互作用位点定位到水稻黄单胞菌RNAP β'亚基N端的四个β'氨基酸残基范围内。该相互作用位点位于开放复合物中靠近启动子间隔区以及延伸复合物中转录泡上游边界的区域，这为一个小蛋白如何通过结合到同一个RNA聚合酶位点来影响转录起始和终止提供了一种可能的解释。