Ståhls Gunilla, Savolainen Eino
Finnish Museum of Natural History, University of Helsinki, P.O. Box 17, FI-00014 Helsinki, Finland.
Mol Phylogenet Evol. 2008 Jan;46(1):82-7. doi: 10.1016/j.ympev.2007.09.009. Epub 2007 Sep 18.
Partial mitochondrial COI sequences (barcoding fragment) were explored for the understanding of the species boundaries of Baetis vernus group taxa (Ephemeroptera, Baetidae) in northern Europe. We sampled all species of this group occurring in Finland, but focused on taxa for which morphological and taxonomical confusion have been most apparent. The sequence matrix comprised 627 nucleotides for 96 specimens, and was analysed using parsimony. Results provided strong evidence that Baetis macani Kimmins and B. vernus Curtis comprise morphologically cryptic but molecularly distinct taxa, as intraspecific uncorrected divergences within haplogroups ranged between 0.3% and 1.4% and interspecific divergences were from 13.1% to 16.5%. These interesting findings prompt for further taxonomic studies of B. vernus taxa using more extensive specimen sampling from the known distributional areas in the Palaearctic/Holarctic region for better understanding of haplotype distributions. We stress the importance of integration of morphological and molecular data, and the necessity to employ additional nuclear DNA sequence data.
为了解北欧细纹长腹蜉蝣类群（蜉蝣目，长腹蜉蝣科）的物种界限，我们研究了部分线粒体COI序列（条形码片段）。我们对芬兰境内该类群的所有物种进行了采样，但重点关注形态学和分类学上混淆最为明显的类群。序列矩阵包含96个标本的627个核苷酸，并采用简约法进行分析。结果提供了有力证据，表明麦氏长腹蜉蝣和细纹长腹蜉蝣是形态上隐秘但分子上不同的类群，因为单倍型类群内的种内未校正分歧在0.3%至1.4%之间，种间分歧在13.1%至16.5%之间。这些有趣的发现促使我们对细纹长腹蜉蝣类群进行进一步的分类学研究，从古北界/全北区已知分布区域采集更广泛的标本样本，以便更好地了解单倍型分布。我们强调整合形态学和分子数据的重要性，以及采用额外核DNA序列数据的必要性。
