Desmyter Stijn, Gosselin Matthias
National Institute for Criminalistics and Criminology, Vilvoordsesteenweg 100, 1120 Brussels, Belgium.
Forensic Sci Int Genet. 2009 Mar;3(2):89-95. doi: 10.1016/j.fsigen.2008.11.002. Epub 2009 Jan 15.
About 50 Chrysomyinae specimens belonging to three forensic relevant species (Chrysomia albiceps, Phormia regina and Protophormia terraenovae) were collected from different geographical locations in Belgium over the last 5 yr. A 304-bp fragment of their mitochondrial COI gene is sequenced. The monophyletic branches of the phylogenetic tree reveal that this marker is suitable for discrimination between these species. The intra versus interspecific variability marks clear threshold levels for DNA barcoding. Nineteen Chrysomyinae specimens, collected from four locations in France, show mitotypes that are identical or at least very similar to the Belgian mitotypes. Considering additional specimens from outside of Europe reveals no intraspecific geographical variation within C. albiceps and P. terraenovae, whereas P. regina is subbranched in a Belgian-French and a USA-Chinese population.
在过去5年中，从比利时不同地理位置收集了约50份属于三个法医相关物种（白头金蝇、丽蝇和新陆原伏蝇）的丽蝇亚科标本。对其线粒体COI基因的304 bp片段进行了测序。系统发育树的单系分支表明，该标记物适用于区分这些物种。种内与种间变异性为DNA条形码标记出了清晰的阈值水平。从法国四个地点收集的19份丽蝇亚科标本显示，其线粒体类型与比利时的线粒体类型相同或至少非常相似。考虑到来自欧洲以外的其他标本，白头金蝇和新陆原伏蝇没有种内地理变异，而丽蝇在比利时 - 法国种群和美国 - 中国种群中形成了亚分支。
