嵌合型1型人类免疫缺陷病毒EcoHIV/NL4-3疫苗诱导的小鼠抗感染保护作用。
Vaccine-induced protection from infection of mice by chimeric human immunodeficiency virus type 1, EcoHIV/NL4-3.
作者信息
Saini Manisha, Hadas Eran, Volsky David J, Potash Mary Jane
机构信息
Molecular Virology Division, St. Luke's Roosevelt Hospital Center, Columbia University Medical Center, 432 West 58th Street, New York, NY 10019, USA.
出版信息
Vaccine. 2007 Dec 17;25(52):8660-3. doi: 10.1016/j.vaccine.2007.10.019. Epub 2007 Oct 31.
Abstract
EcoHIV/NL4-3 is a chimeric human immunodeficiency virus type 1 (HIV-1) that can productively infect mice. This study tests the utility of EcoHIV/NL4-3 infection to reveal protective immune responses to an HIV-1 vaccine. Immunocompetent mice were first immunized with VRC 4306 which encodes subtype B consensus sequences of gag, pol, and nef and then were infected by EcoHIV/NL4-3. Anti-Gag antibodies were sampled during immunization and infection. The extent of EcoHIV/NL4-3 infection in spleen cells and peritoneal macrophages was determined by quantitative real-time PCR (QPCR). Although antibody titres were not significantly different in control and vaccinated groups, VRC 4306 immunization induced protective responses that significantly reduced virus burden in both lymphocyte and macrophage compartments. These results indicate that EcoHIV/NL4-3 infection can be controlled by HIV-1 vaccine-induced responses, introducing a small animal model to test vaccine efficacy against HIV-1 infection.
摘要
EcoHIV/NL4-3是一种嵌合的1型人类免疫缺陷病毒（HIV-1），能够有效感染小鼠。本研究测试了EcoHIV/NL4-3感染在揭示针对HIV-1疫苗的保护性免疫反应方面的效用。具有免疫活性的小鼠首先用编码gag、pol和nef的B亚型共有序列的VRC 4306进行免疫，然后感染EcoHIV/NL4-3。在免疫和感染期间采集抗Gag抗体。通过定量实时PCR（QPCR）确定脾脏细胞和腹腔巨噬细胞中EcoHIV/NL4-3的感染程度。尽管对照组和疫苗接种组的抗体滴度没有显著差异，但VRC 4306免疫诱导了保护性反应，显著降低了淋巴细胞和巨噬细胞区室中的病毒载量。这些结果表明，EcoHIV/NL4-3感染可被HIV-1疫苗诱导的反应所控制，从而引入了一种小型动物模型来测试针对HIV-1感染的疫苗效力。
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