Goldstein N O, Pardoe I U, Burness A T
J Gen Virol. 1976 May;31(2):271-6. doi: 10.1099/0022-1317-31-2-271.
About 80% of the RNA molecules extracted from encephalomyocarditis (EMC) virus were bound by oligo(dT)-cellulose under conditions which bind poly(A) but not poly(C) nor ribosomal RNA. This shows that most EMC virus RNA molecules contain a poly(A) tract. Both bound and unbound fractions contained RNA molecules of apparently the same size when examined by sucrose gradient sedimentation, but the bound fraction contained an adenylic acid-rich segment of about 20 nucleotides long, whereas the unbound RNA did not. The bound RNA had 200 times the specific infectivity of the unbound RNA which suggests that the poly(A) tract present in EMC virus RNA is required for infectivity.
从脑心肌炎（EMC）病毒中提取的RNA分子，约80%在能结合多聚腺苷酸（poly(A)）但不能结合多聚胞苷酸（poly(C)）和核糖体RNA的条件下，被寡聚（dT）纤维素所结合。这表明大多数EMC病毒RNA分子含有一个多聚腺苷酸尾。通过蔗糖梯度沉降分析，结合部分和未结合部分所含的RNA分子大小明显相同，但结合部分含有一段约20个核苷酸长的富含腺苷酸的片段，而未结合的RNA则没有。结合的RNA的比感染力是未结合RNA的200倍，这表明EMC病毒RNA中存在的多聚腺苷酸尾是感染性所必需的。
