脑心肌炎病毒RNA中聚腺苷酸序列的大小。

Size of the poly(A) sequences in encephalomyocarditis virus RNA.

作者信息

Giron M L, Logeat F, Hanania N, Fossar N, Huppert J

出版信息

Intervirology. 1975;6(6):367-71. doi: 10.1159/000149493.

Abstract

Poly(A) sequences were demonstrated in the RNA of encephalomyocarditis (EMC) virus by affinity chromatography on poly(U)-Sepharose, treatment with RNases, and determination of the ability of the RNA to prime for reverse transcription. The size of the poly(A) sequences was estimated as 10-20 nucleotides in length by determination of the fraction resistant to pancreatic RNases plus T1 RNases, followed by analysis of electrophoretic mobility on polyacrylamide gels and of the AMP-to-adenosine ratio after alkaline hydrolysis. The poly(A) sequences are located at the 3' end of the RNA as shown by mild digestion by snake venom phosphodiesterase.

摘要

通过在聚（U）-琼脂糖上进行亲和层析、用核糖核酸酶处理以及测定RNA引发逆转录的能力，在脑心肌炎（EMC）病毒的RNA中证实了聚（A）序列。通过测定对胰核糖核酸酶加T1核糖核酸酶有抗性的部分，随后分析在聚丙烯酰胺凝胶上的电泳迁移率以及碱性水解后的AMP与腺苷的比率，估计聚（A）序列的大小为10 - 20个核苷酸长。如用蛇毒磷酸二酯酶温和消化所示，聚（A）序列位于RNA的3'末端。