Zhou Guo Shun, Su Zhong Yuan, Cai Yu Rong, Liu Yu Kan, Dai Li Cheng, Tang Rui Kang, Zhang Ming
Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang Province, China.
Biomed Mater Eng. 2007;17(6):387-95.
It is suggested that nanophase hydroxyapatite (nHAP) might have advantages over conventional hydroxyapatite (cHAP) as a biomaterial for bone regeneration. To be a satisfactory candidate for bone tissue engineering, it is important to support the growth and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). The purpose of this study is to determine whether nHAP as cell growth substrata could give better support for attachment, proliferation and osteogenic differentiation of BMSCs than cHAP.
nHAP and cHAP films were prepared as the substrata for the cell growth. BMSCs obtained from rabbit were seeded on the films. Attachment, proliferation and osteogenic differentiation of BMSCs on the two kinds of films were evaluated.
Cell attachment ratio on nHAP films was significantly higher than that on cHAP films. Doubling time on nHAP films was significantly shorter than that on cHAP films. Amount of total proteins detected from cells cultured on nHAP films was significantly higher than that on cHAP films. However, alkaline phosphatase activity and osteocalcin content of the two groups showed no significant difference.
nHAP films favored cell attachment and proliferation but not osteogenic differentiation of BMSCs compared with cHAP films.
有人提出，作为用于骨再生的生物材料，纳米相羟基磷灰石（nHAP）可能比传统羟基磷灰石（cHAP）具有优势。要成为骨组织工程的理想候选材料，支持骨髓间充质干细胞（BMSC）的生长和分化很重要。本研究的目的是确定作为细胞生长基质的nHAP是否比cHAP能更好地支持BMSC的附着、增殖和成骨分化。
制备nHAP和cHAP薄膜作为细胞生长的基质。将从兔子获得的BMSC接种到薄膜上。评估BMSC在两种薄膜上的附着、增殖和成骨分化情况。
nHAP薄膜上的细胞附着率显著高于cHAP薄膜。nHAP薄膜上的倍增时间显著短于cHAP薄膜。在nHAP薄膜上培养的细胞中检测到的总蛋白量显著高于cHAP薄膜。然而，两组的碱性磷酸酶活性和骨钙素含量无显著差异。
与cHAP薄膜相比，nHAP薄膜有利于BMSC的细胞附着和增殖，但不利于其成骨分化。
