Kilic Abdullah, Senses Zeynep, Kurekci A Emin, Aydogan Hakan, Sener Kenan, Kismet Erol, Basustaoglu A Celal
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Adademy, School of Medicine, Ankara, Turkey.
Jpn J Infect Dis. 2007 Nov;60(6):394-6.
Nosocomial Sphingomonas paucimobilis infections can arise from contaminated water and the contaminated hands of hospital staff. Within a 1-month period, we isolated six S. paucimobilis strains, including four from blood cultures of four patients and two from hospital environment specimens including tap water and a bathtub in a hemato/oncology unit. We described here these strains' molecular epidemiological analyses by pulsed-field gel electrophoresis (PFGE) and antibiotic susceptibilities by E-test. Although clinical and environmental isolates yielded three different antibiotic resistances and PFGE patterns, all four clinical strains had an identical pattern by both methods. Thus, the isolated clinical strain clone could be traced neither to health care workers nor to environmental samples. It was concluded that S. paucimobilis strains can cause outbreaks in hemato/oncology units. We did not demonstrate genetic relatedness between clinical and environmental isolates by PFGE, but did find PFGE a useful identification technique for epidemiological investigation.
医院内少动鞘氨醇单胞菌感染可能源于受污染的水以及医院工作人员被污染的手。在1个月的时间内，我们分离出6株少动鞘氨醇单胞菌，其中4株来自4名患者的血培养，2株来自血液/肿瘤科的医院环境标本，包括自来水和一个浴缸。我们在此描述了通过脉冲场凝胶电泳（PFGE）对这些菌株进行的分子流行病学分析以及通过E-test法检测的抗生素敏感性。尽管临床分离株和环境分离株产生了三种不同的抗生素耐药性和PFGE图谱，但所有4株临床菌株通过这两种方法都具有相同的图谱。因此，分离出的临床菌株克隆既无法追溯到医护人员，也无法追溯到环境样本。得出的结论是，少动鞘氨醇单胞菌菌株可在血液/肿瘤科引发感染暴发。我们通过PFGE未证明临床分离株和环境分离株之间存在遗传相关性，但确实发现PFGE是一种用于流行病学调查的有用鉴定技术。
