Abdiu O, Van Setten G
Laboratory of DOHF, St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
Ophthalmic Res. 2008;40(1):16-8. doi: 10.1159/000111153. Epub 2007 Nov 20.
BACKGROUND/PURPOSE: Pigment-epithelium-derived factor (PEDF) is of major importance to prevent neovascularization of the retina. Recently found to occur in the cornea and conjunctival tissue, its presence in tears has this far not been reported. Initial results, based on the analysis of diluted tear fluid samples, even indicated the absence of this factor in human tears. Considering the clinical importance of PEDF as regulator of corneal vascularization, we investigated undiluted human tears.
Samples of 18 healthy individuals were collected and analyzed using a commercial ELISA. Samples were also collected from 5 patients with pterygium and as a positive control 2 samples of subretinal fluid from patients following retinal detachment surgery.
PEDF concentrations were below the detection limit (i.e. <0.1 ng/ml) in the majority of samples from the healthy individuals. However, PEDF was discovered in 3 of the samples taken, with significant concentrations of 2, 32 and 53 ng/ml. In the group of 5 patients with pterygium, there were no detectable concentrations of PEDF.
PEDF may be found in measurable amounts in human tear fluid of healthy individuals and may therefore play a role in the effects and regulation of PEDF at the ocular surface.
背景/目的：色素上皮衍生因子（PEDF）对于预防视网膜新生血管形成至关重要。最近发现它存在于角膜和结膜组织中，但迄今为止尚未有其在泪液中存在的报道。基于对稀释泪液样本的分析，初步结果甚至表明人泪液中不存在该因子。鉴于PEDF作为角膜血管生成调节因子的临床重要性，我们对未稀释的人泪液进行了研究。
收集了18名健康个体的样本，并使用商业酶联免疫吸附测定（ELISA）进行分析。还从5名患有翼状胬肉的患者中采集了样本，并作为阳性对照，采集了2例视网膜脱离手术后患者的视网膜下液样本。
大多数健康个体的样本中，PEDF浓度低于检测限（即<0.1 ng/ml）。然而，在所采集的样本中有3份检测到了PEDF，其浓度分别为2、32和53 ng/ml，浓度较高。在5名翼状胬肉患者组中，未检测到PEDF浓度。
在健康个体的人泪液中可能会发现可测量量的PEDF，因此它可能在眼表PEDF的作用和调节中发挥作用。
