Khamidov D Kh, Mirakhmedov A K, Aripdzhanov A A, Sagatova G A, Azimova Sh S
Tsitologiia. 1991;33(4):84-8.
Intracellular distribution of labeled cotoran was studied. 3H-cotoran was shown to penetrate through the nuclear membrane to accumulate uneventfully in the intranuclear components. An insignificant amount of 3H-cotoran was associated with the nucleoplasm and the outer nuclear membrane. At the same time, essential radioactivity was observed in the proteins of the nuclear matrix (up to 30%) and in non-histone proteins of chromatin (up to 60%). Acception of 3H-cotoran on metaphase chromosomes of cultured cells as well as specificity of cotoran binding with non-histone proteins of chromatin in vivo and in vitro was studied by radioautography. It was revealed that cotoran was translocated into the interphase nuclei to be accepted by metaphase chromosomes of the HeLa line cells and fibroblasts in human embryo, and specifically, in receptor-like manner, bound to chromatin proteins.
研究了标记的考托兰的细胞内分布。结果显示，³H-考托兰能够穿透核膜并在核内成分中平稳积累。仅有少量³H-考托兰与核质和核外膜相关联。与此同时，在核基质蛋白（高达30%）和染色质非组蛋白（高达60%）中观察到显著的放射性。通过放射自显影术研究了³H-考托兰在培养细胞中期染色体上的摄取情况以及考托兰在体内和体外与染色质非组蛋白结合的特异性。结果表明，考托兰转移至间期细胞核，被人胚胎的HeLa系细胞和成纤维细胞的中期染色体摄取，并且以类似受体的方式特异性地与染色质蛋白结合。
