依布硒啉对亚硒酸钠诱导的大鼠实验性白内障的保护作用。

Protective effects of ebselen on sodium-selenite-induced experimental cataract in rats.

机构信息

Department of Ophthalmology, School of Medicine, Fırat University, Elazığ, Turkey.

出版信息

J Cataract Refract Surg. 2012 Dec;38(12):2160-6. doi: 10.1016/j.jcrs.2012.07.022. Epub 2012 Sep 19.

DOI:10.1016/j.jcrs.2012.07.022

PMID:22999516

Abstract

PURPOSE

To determine whether ebselen has a protective effect or antioxidative potential in a sodium-selenite-induced experimental cataract model.

SETTING

Fırat University, Elazığ, Turkey.

DESIGN

Experimental study.

METHODS

Twenty-one Sprague-Dawley rat pups were randomly divided into a control group, a sodium-selenite-induced-cataract group, and an ebselen-treated group; each group contained 7 rat pups. Rats in the control group received dimethyl sulfoxide (DMSO) intraperitoneally only and rats in the sodium-selenite-induced-cataract group received 30 nmol/g body weight sodium selenite subcutaneously and DMSO intraperitoneally 10 days postpartum. Rats in the ebselen group received 30 nmol/g body weight sodium selenite subcutaneously 10 days postpartum and were treated with 5 mg/kg body weight ebselen once a day for 4 consecutive days. Cataract development was assessed weekly for 3 weeks by slitlamp examination and graded using a scale. Reduced glutathione (GSH), total nitrite, and malondialdehyde (MDA) levels in lens supernatants were measured at the end of 3 weeks.

RESULTS

In the control group, all lenses were clear. In the ebselen-treated group, the mean cataract stage was significantly lower than in the sodium-selenite-induced-cataract group (P = .022). The GSH levels were significantly lower in the sodium-selenite-induced-cataract group than in the control and ebselen groups (P < .001). The MDA levels were lower in the ebselen group than in the sodium-selenite-induced-cataract group (P < .001). The mean total nitrite level was significantly lower in the sodium-selenite-induced-cataract group than in the ebselen group (P = .001).

CONCLUSIONS

Ebselen had a protective effect on cataract development in a sodium-selenite-induced experimental model. The protective effect of ebselen appears to be due to inhibition of oxidative stress.

FINANCIAL DISCLOSURE

No author has a financial or proprietary interest in any material or method mentioned.

摘要

目的

研究依布硒啉（ebselen）在亚硒酸钠诱导的实验性白内障模型中是否具有保护作用或抗氧化潜能。

背景

依布硒啉是一种有机硒化合物，具有抗氧化和抗炎作用。它已被用于治疗多种疾病，包括糖尿病、心血管疾病和自身免疫性疾病。在白内障的形成过程中，氧化应激被认为是一个重要的致病因素。依布硒啉的抗氧化作用可能与其能够清除自由基和抑制脂质过氧化有关。

地点

土耳其埃尔祖鲁姆法提大学。

设计

实验研究。

方法

21 只斯普拉格-道利（Sprague-Dawley）幼鼠随机分为对照组、亚硒酸钠诱导性白内障组和依布硒啉治疗组，每组 7 只幼鼠。对照组仅接受二甲基亚砜（DMSO）腹腔内注射，亚硒酸钠诱导性白内障组产后第 10 天接受 30nmol/g 体重的亚硒酸钠皮下注射和腹腔内注射 DMSO，依布硒啉组产后第 10 天接受 30nmol/g 体重的亚硒酸钠皮下注射，并每天接受 5mg/kg 体重的依布硒啉治疗，连续 4 天。在 3 周内每周通过裂隙灯检查评估白内障的发展情况，并使用量表进行分级。在 3 周末测量晶状体上清液中的还原型谷胱甘肽（GSH）、总亚硝酸盐和丙二醛（MDA）水平。

结果

对照组所有晶状体均清晰。依布硒啉治疗组白内障分期明显低于亚硒酸钠诱导性白内障组（P=0.022）。与对照组和依布硒啉组相比，亚硒酸钠诱导性白内障组的 GSH 水平显著降低（P<0.001）。与亚硒酸钠诱导性白内障组相比，依布硒啉组的 MDA 水平较低（P<0.001）。与依布硒啉组相比，亚硒酸钠诱导性白内障组的总亚硝酸盐水平显著降低（P=0.001）。