Ostlund Maria Rotzén, Lindell Annika Tiveljung, Stenler Sofia, Riedel Hilde M, Wirgart Benita Zweygberg, Grillner Lena
Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
J Med Virol. 2008 Jan;80(1):159-67. doi: 10.1002/jmv.21066.
The epidemiology and genetic variability of circulating respiratory syncytial virus (RSV) strains in Stockholm during the season 2002-2003 were studied in consecutive RSV isolates derived from respiratory samples and diagnosed in the laboratory. Two hundred thirty-four viruses were sequenced. The samples were mainly from children under 1 year old (79%). The phylogeny of the N-terminal part of the G gene was studied after amplification and sequencing. One hundred fifty-two viruses belonged to subgroup B and 82 to subgroup A. The subgroup A viruses could be further divided into genotypes GA2 (25) and GA5 (57) and the subgroup B viruses into GB3 (137) and SAB1 (15) strains. These strains clustered with subgroup A and subgroup B strains from Kenya from the same period, as well as with strains from Great Britain from 1995 to 1998. The dominance of subgroup B strains in Stockholm during 2002-2003 is in agreement with findings from other parts of the world during the same years. Only two genotypes of subgroup A, GA2 and GA5, were circulating during this time, and GA2 has been circulating in Sweden for more than 20 years. Consecutive strains from the same individual displayed no variability in the sequenced region, which was also true of strains that had been passaged in cell cultures.
对2002 - 2003年期间在斯德哥尔摩从呼吸道样本中分离并经实验室诊断的呼吸道合胞病毒（RSV）连续毒株，研究了其流行病学和基因变异性。对234株病毒进行了测序。样本主要来自1岁以下儿童（79%）。扩增和测序后研究了G基因N端部分的系统发育。152株病毒属于B亚组，82株属于A亚组。A亚组病毒可进一步分为GA2基因型（25株）和GA5基因型（57株），B亚组病毒分为GB3基因型（137株）和SAB1基因型（15株）。这些毒株与同期来自肯尼亚的A亚组和B亚组毒株以及1995年至1998年来自英国的毒株聚集在一起。2002 - 2003年期间斯德哥尔摩B亚组毒株占优势，这与同一年世界其他地区的研究结果一致。在此期间，A亚组仅两种基因型GA2和GA5在传播，且GA2已在瑞典传播超过20年。来自同一个体的连续毒株在测序区域没有变异性，在细胞培养中传代的毒株也是如此。
