Bresson-Hadni S, Monnot-Jacquard B, Racadot E, Lenys D, Miguet J P, Vuitton D A
Groupe de Recherche sur l'Echinococcose Alvéolaire, Faculté de Médecine et de Pharmacie, University of Franche-Comté, Besançon, France.
Eur Cytokine Netw. 1991 Nov-Dec;2(5):339-44.
Cellular immunity plays a key role in the defence against the larva of the cestode Echinococcus multilocularis. This larva is responsible for alveolar echinococcosis (AE) of the liver, a rare parasitic disease which occurs in endemic areas including European alpine countries, Alaska, the USSR, Western China and Northern Japan. We have shown a marked decrease of the CD8+ T-cell population in the blood and we have described an infiltrate composed mainly of activated CD8+ T-cells in the liver lesions of most patients with AE. In this study, we assessed the serum level of soluble IL-2-receptor (sIL-2R) and CD8 (sCD8) in 37 patients (23 men, 14 women, mean age 59.5 yrs) with a histologically proven AE. The results, obtained using sandwich ELISA, were compared to those of healthy controls and correlated to parameters evaluating the severity of the disease. The mean serum levels of sIL-2R were significantly higher in AE patients than in controls. There was a significant correlation between sIL-2R levels and both the volume of parasitic lesions and a calculated index of severity of the disease. The mean serum levels of sCD8 did not differ significantly from the values obtained in controls. These results indicate that the infiltration of the liver by CD8+ T-lymphocytes is not associated with an increased release of sCD8 into the serum. The circulating levels of sIL-2R appear to reflect the extent as well as the severity of the disease. Immunostaining of the cells of the periparasitic granuloma suggests that the cell origin of the sIL-2R could be macrophages rather than T-lymphocytes.
细胞免疫在抵御多房棘球绦虫幼虫的过程中发挥着关键作用。这种幼虫是导致肝脏泡型包虫病（AE）的病原体，AE是一种罕见的寄生虫病，发生在包括欧洲高山国家、阿拉斯加、苏联、中国西部和日本北部在内的流行地区。我们已经发现血液中CD8 + T细胞数量显著减少，并且描述了大多数AE患者肝脏病变中主要由活化的CD8 + T细胞组成的浸润情况。在本研究中，我们评估了37例经组织学证实为AE的患者（23名男性，14名女性，平均年龄59.5岁）血清中可溶性白细胞介素-2受体（sIL-2R）和CD8（sCD8）的水平。使用夹心酶联免疫吸附测定法获得的结果与健康对照者的结果进行了比较，并与评估疾病严重程度的参数相关联。AE患者血清中sIL-2R的平均水平显著高于对照组。sIL-2R水平与寄生虫病变体积和计算得出的疾病严重程度指数之间存在显著相关性。sCD8的平均血清水平与对照组的值无显著差异。这些结果表明，CD8 + T淋巴细胞对肝脏的浸润与血清中sCD8释放增加无关。sIL-2R的循环水平似乎反映了疾病的程度和严重程度。寄生虫周围肉芽肿细胞的免疫染色表明，sIL-2R的细胞来源可能是巨噬细胞而非T淋巴细胞。
