Wang Xiao-Yan, Lan Yu, He Wen-Yan, Zhang Lei, Yao Hui-Yu, Hou Chun-Mei, Tong Ying, Liu Yuan-Lin, Yang Guan, Liu Xiao-Dan, Yang Xiao, Liu Bing, Mao Ning
Department of Cell Biology, Institute of Basic Medical Sciences, Beijing, China.
Blood. 2008 Feb 15;111(4):2436-43. doi: 10.1182/blood-2007-07-099333. Epub 2007 Nov 28.
Mesenchymal stem cells (MSCs) are multipotent stem cells that can generate various microenvironment components in bone marrow, ensuring a precise control over self-renewal and multilineage differentiation of hematopoietic stem cells. Nevertheless, their spatiotemporal correlation with embryonic hematopoiesis remains rudimentary, particularly in relation to the human being. Here, we reported that human aorta-gonad-mesonephros (AGM) resided with bona fide MSCs. They were highly proliferative as fibroblastoid population bearing uniform surface markers (CD45(-), CD34(-), CD105(+), CD73(+), CD29(+), and CD44(+)), expressed pluripotential molecules Oct-4 and Nanog, and clonally demonstrated trilineage differentiation capacity (osteocytes, chondrocytes, and adipocytes). The frequency and absolute number of MSCs in aorta plus surrounding mesenchyme (E26-E27) were 0.3% and 164, respectively. Moreover, they were functionally equivalent to MSCs from adult bone marrow, that is, supporting long-term hematopoiesis and suppressing T-lymphocyte proliferation in vitro. In comparison, the matching yolk sac contained bipotent mesenchymal precursors that propagated more slowly and failed to generate chondrocytes in vitro. Together with previous knowledge, we propose that a proportion of MSCs initially develop in human AGM prior to their emergence in embryonic circulation and fetal liver.
间充质干细胞（MSCs）是多能干细胞，能够在骨髓中生成各种微环境成分，确保对造血干细胞的自我更新和多谱系分化进行精确控制。然而，它们与胚胎造血的时空相关性仍处于初步阶段，尤其是在人类方面。在此，我们报告人类主动脉-性腺-中肾（AGM）区域存在真正的间充质干细胞。它们作为具有统一表面标志物（CD45(-)、CD34(-)、CD105(+)、CD73(+)、CD29(+)和CD44(+)）的成纤维样细胞群体具有高度增殖能力，表达多能性分子Oct-4和Nanog，并通过克隆展示三谱系分化能力（成骨细胞、软骨细胞和脂肪细胞）。主动脉加周围间充质（E26-E27）中间充质干细胞的频率和绝对数量分别为0.3%和164。此外，它们在功能上等同于来自成人骨髓的间充质干细胞，即在体外支持长期造血并抑制T淋巴细胞增殖。相比之下，匹配的卵黄囊中含有双能间充质前体细胞，其增殖较慢且在体外无法生成软骨细胞。结合先前的知识，我们提出一部分间充质干细胞最初在人类AGM区域发育，然后才出现在胚胎循环和胎儿肝脏中。
