Fecka Izabela, Turek Sebastian
Department of Pharmacognosy, Wrocław Medical University, Poland.
J Agric Food Chem. 2007 Dec 26;55(26):10908-17. doi: 10.1021/jf072284d. Epub 2007 Dec 4.
Chromatographic techniques (HPLC and HPTLC) were used for qualitative and quantitative determination of eriocitrin, luteolin 7-O-rutinoside, luteolin 7-O-beta-glucuronide, lithospermic acid, rosmarinic acid, and methyl rosmarinate together with other known compounds in commercial herbal teas from the Lamiaceae family: peppermint leaf (Menthae piperitae folium), melissa leaf (Melissae folium), and sage leaf (Salviae officinalis folium). Contents of analyzed compounds in infusions, the most popular forms, were established using a C18 column with acetonitrile-water-formic acid as a mobile phase. The HPLC method was validated for linearity, precision, and accuracy. Luteolin 7-O-beta-glucuronide and lithospermic acid were identified as new Mentha x piperita compounds. The investigated herbal teas delivered polyphenols in high amounts, up to 182.2 mg for the infusion of one peppermint tea bag.
采用色谱技术（高效液相色谱法和高效薄层色谱法）对迷迭香酸、迷迭香酸甲酯以及异柠檬酸、木犀草素7 - O - 芸香糖苷、木犀草素7 - O - β - 葡萄糖醛酸、紫草酸等其他已知化合物进行定性和定量测定，这些化合物存在于唇形科的商业凉茶中：薄荷叶（薄荷叶）、蜜蜂花叶（蜜蜂花叶）和鼠尾草叶（鼠尾草叶）。使用以乙腈 - 水 - 甲酸为流动相的C18柱，测定了最常见形式的浸液中分析化合物的含量。对高效液相色谱法的线性、精密度和准确度进行了验证。木犀草素7 - O - β - 葡萄糖醛酸和紫草酸被鉴定为新的薄荷属化合物。所研究的凉茶含有大量多酚，一袋薄荷茶浸液中多酚含量高达182.2毫克。
