连续和脉冲超声对培养的（AL）人-仓鼠杂交细胞无诱变作用。

Absence of mutagenic effects of continuous and pulsed ultrasound in cultured (AL) human-hamster hybrid cells.

作者信息

Ritenour E R, Braaton M, Harrison G H, Ueno A, Gadd M, Manco-Johnson M, Parker R, Shih S, Waldren C A

机构信息

Dept. of Radiology, University of Colorado School of Medicine, Denver 80262.

出版信息

Ultrasound Med Biol. 1991;17(9):921-30. doi: 10.1016/0301-5629(91)90086-c.

DOI:10.1016/0301-5629(91)90086-c

PMID:1805481

Abstract

Mutagenic effects of continuous and pulsed ultrasound were looked for using an in vitro assay system, the AL hybrid, that is up to 100 times more sensitive for mutagens such as x-rays and neutrons than the assays used previously to evaluate ultrasound. Cells in suspension in rotated plastic test tubes were insonated with continuous wave ultrasound at 1 MHz, ISPTP = 0.62-40 W/cm2 for 0-40 min. Cells attached in the central region of culture flasks received pulsed exposures at fc = 2.5 MHz, PRF = 1 kHz, 2 and 8 cycles per pulse, with p- = 1.2 MPa (ISPTA = 31-180 mW/cm2) for 0-30 min. Although these exposures were cytotoxic (the plating efficiency was decreased to approximately 65% by the highest doses), induction of mutation, if any occurred, was less than would be expected in this test system from 10-30 cGy of x-ray.

摘要

利用一种体外检测系统——AL杂种细胞，研究连续超声和脉冲超声的诱变效应。该系统对X射线和中子等诱变剂的敏感性比以前用于评估超声的检测方法高出100倍。将悬浮在旋转塑料试管中的细胞用1MHz的连续波超声进行辐照，ISPTP = 0.62 - 40W/cm²，持续0 - 40分钟。附着在培养瓶中心区域的细胞接受脉冲辐照，频率fc = 2.5MHz，脉冲重复频率PRF = 1kHz，每个脉冲2和8个周期，声压p- = 1.2MPa（ISPTA = 31 - 180mW/cm²），持续0 - 30分钟。尽管这些辐照具有细胞毒性（最高剂量可使接种效率降至约65%），但如果发生诱变，其诱变率低于在该检测系统中10 - 30cGy X射线照射所预期的诱变率。