Diniz Yeda S, Santos Priscila P, Assalin Heloisa B, Souza Gisele A, Rocha Katiucha K H R, Ebaid Geovana M X, Seiva Fabio R F, Amauchi Juliana F, Novelli Filho José Luiz V B, Novelli Ethel L B
Department of Chemistry and Biochemistry, Institute of Biological Sciences, São Paulo State University, UNESP, 18618-000, Botucatu, São Paulo, Brazil.
Eur J Pharmacol. 2008 Jan 28;579(1-3):318-25. doi: 10.1016/j.ejphar.2007.11.008. Epub 2007 Nov 13.
Studies on conjugated linoleic acid ingestion and its effect on cardiac tissue are necessary for the safe utilization of this compound as supplement for weight loss. Male Wistar 24-rats were divided into four groups (n=6):(C)given standard chow, water and 0.5 ml saline, twice a week by gavage; (C-CLA)receiving standard chow, water and 0.5 ml of conjugated linoleic acid, twice a week, by gavage; (S)given standard chow, saline by gavage, and 30% sucrose in its drinking water; (S-CLA)receiving standard chow, 30% sucrose in its drinking water and conjugated linoleic acid. After 42 days of treatment S rats had obesity with increased abdominal-circumference, dyslipidemia, oxidative stress and myocardial lower citrate synthase(CS) and higher lactate dehydrogenase(LDH) activities than C. Conjugated linoleic acid had no effects on morphometric parameters in C-CLA, as compared to C, but normalized morphometric parameters comparing S-CLA with S. There was a negative correlation between abdominal adiposity and resting metabolic rate. Conjugated linoleic acid effect, enhancing fasting-VO(2)/surface area, postprandial-carbohydrate oxidation and serum lipid hydroperoxide resembled to that of the S group. Conjugated linoleic acid induced cardiac oxidative stress in both fed conditions, and triacylglycerol accumulation in S-CLA rats. Conjugated linoleic acid depressed myocardial LDH comparing C-CLA with C, and beta-hydroxyacyl-coenzyme-A dehydrogenase/CS ratio, comparing S-CLA with S. In conclusion, dietary conjugated linoleic acid supplementation for weight loss can have long-term effects on cardiac health. Conjugated linoleic acid, isomers c9, t11 and t10, c12c9,t11" and "t10,c12" were changed to "c9, t11" and "t10, c12", respectively. Please check if appropriate.--> presented undesirable pro-oxidant effect and induced metabolic changes in cardiac tissue. Nevertheless, despite its effect on abdominal adiposity in sucrose-rich diet condition, conjugated linoleic acid may be disadvantageous because it can lead to oxidative stress and dyslipidemic profile.
为了安全地将共轭亚油酸作为减肥补充剂使用，有必要对其摄入情况及其对心脏组织的影响进行研究。将雄性Wistar大鼠分为四组（n = 6）：（C）组给予标准饲料、水和0.5毫升生理盐水，每周两次经口灌胃；（C - CLA）组给予标准饲料、水和0.5毫升共轭亚油酸，每周两次经口灌胃；（S）组给予标准饲料，经口灌胃生理盐水，并在饮用水中添加30%蔗糖；（S - CLA）组给予标准饲料，饮用水中添加30%蔗糖并给予共轭亚油酸。治疗42天后，S组大鼠出现肥胖，腹围增加、血脂异常、氧化应激，与C组相比，心肌柠檬酸合酶（CS）活性降低，乳酸脱氢酶（LDH）活性升高。与C组相比，共轭亚油酸对C - CLA组的形态学参数无影响，但将S - CLA组与S组相比，可使形态学参数恢复正常。腹部肥胖与静息代谢率之间呈负相关。共轭亚油酸的作用，即提高空腹VO₂/体表面积、餐后碳水化合物氧化和血清脂质氢过氧化物，与S组相似。在两种进食条件下，共轭亚油酸均诱导心脏氧化应激，并使S - CLA组大鼠出现三酰甘油蓄积。与C组相比，共轭亚油酸使C - CLA组心肌LDH降低，与S组相比，使β - 羟酰基辅酶A脱氢酶/CS比值降低。总之，饮食中补充共轭亚油酸用于减肥可能会对心脏健康产生长期影响。共轭亚油酸的异构体c9，t11和t10，c12分别改为“c9，t11”和“t10，c12”。请检查是否合适。共轭亚油酸呈现出不良的促氧化作用，并在心脏组织中诱导代谢变化。然而，尽管共轭亚油酸在富含蔗糖的饮食条件下对腹部肥胖有影响，但它可能是不利的，因为它会导致氧化应激和血脂异常。
