Singh Shamjeet, Netticadan Thomas, Ramdath D Dan
Department of Pre-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and Tobago West Indies.
Canadian Centre for Agri-Food Research in Health and Medicine, 351 Taché Avenue, Winnipeg, Manitoba Canada.
Genes Nutr. 2016 Mar 17;11:8. doi: 10.1186/s12263-016-0516-4. eCollection 2016.
Insulin resistance is associated with impaired cardiac function, but the underlying molecular abnormalities are largely unexplained. Bilberry anthocyanin (BAcn) may be protective, as it appears to potentiate insulin action.
Rats were randomly allocated to control, sucrose-fed (SF) or sucrose-fed + BAcn diets (SF-A) for 15 weeks. Cardiac insulin signalling genes and proteins were quantified using reverse transcription quantitative real-time polymerase chain reaction and western blots.
Glucose tolerance was not different with treatment. SF showed lower (p < 0.05) ferric reducing antioxidant power, which increased with BAcn. SF resulted in significantly decreased (p < 0.05) expression of 10 genes: acetyl-coenzyme A carboxylase alpha; V-Akt murine thymoma viral oncogene homolog 1; Bcl2-like 1; cytosine-cytosine-adenosine-adenosine-thymidine/enhancer binding protein; FK506 binding protein 12-rapamycin associated; glycerol-3-phosphate dehydrogenase 1 (soluble); solute carrier family 2 (facilitated glucose transporter), member 1, 4; hexokinase 2; and thyroglobulin. SF-A prevented these changes. Compared to SF-A, SF up-regulated (p < 0.05) complement factor D and phosphoinositide-3-kinase, regulatory subunit1 (α); sterol regulatory element binding transcription factor 1 was down-regulated (p < 0.05). SF increased (p < 0.05) cardiac phospholamban and decreased phosphorylated troponin I, which were not attenuated by BAcn. Compared to control or SF, SF-A resulted in significantly lower (p < 0.05) 5'-AMP-activated protein kinase.
SF lowered antioxidant capacity and changed the expression of insulin signalling genes, which were modulated by BAcn.
胰岛素抵抗与心脏功能受损有关，但其潜在的分子异常在很大程度上尚不清楚。越橘花青素（BAcn）可能具有保护作用，因为它似乎能增强胰岛素作用。
将大鼠随机分为对照组、蔗糖喂养（SF）组或蔗糖喂养 + BAc n组（SF - A），持续15周。使用逆转录定量实时聚合酶链反应和蛋白质印迹法对心脏胰岛素信号基因和蛋白质进行定量分析。
不同处理组之间的葡萄糖耐量无差异。SF组的铁还原抗氧化能力较低（p < 0.05），而BAcn可使其升高。SF组导致10个基因的表达显著降低（p < 0.05）：乙酰辅酶A羧化酶α；V - Akt小鼠胸腺瘤病毒癌基因同源物1；Bcl2样蛋白1；胞嘧啶 - 胞嘧啶 - 腺苷 - 腺苷 - 胸腺嘧啶/增强子结合蛋白；FK506结合蛋白12 - 雷帕霉素相关蛋白；甘油 - 3 - 磷酸脱氢酶1（可溶性）；溶质载体家族2（促进性葡萄糖转运蛋白），成员1、4；己糖激酶2；以及甲状腺球蛋白。SF - A组可预防这些变化。与SF - A组相比，SF组上调了（p < 0.05）补体因子D和磷酸肌醇 - 3 - 激酶调节亚基1（α）；固醇调节元件结合转录因子1下调（p < 0.05）。SF组增加了（p < 0.05）心脏受磷蛋白，降低了磷酸化肌钙蛋白I，而BAcn并未减弱这些变化。与对照组或SF组相比，SF - A组的5'-AMP活化蛋白激酶显著降低（p < 0.05）。
SF降低了抗氧化能力并改变了胰岛素信号基因的表达，而BAcn可对其进行调节。
