Lai Xintian, Cao Lixiang, Tan Hongming, Fang Shu, Huang Yali, Zhou Shining
State Key Laboratory for Biocontrol and Department of Biochemistry, College of Life Sciences, Zhongshan (Sun Yatsen) University, Guangzhou, People's Republic of China.
ISME J. 2007 Dec;1(8):756-62. doi: 10.1038/ismej.2007.51. Epub 2007 Oct 18.
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71-97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea.
为阐明南海含甲烷水合物深海海洋沉积物中的真菌多样性，对五个不同沉积物DNA样本的rRNA基因内部转录间隔区（ITS）进行了扩增和系统发育分析。共构建了五个ITS文库，通过核糖体DNA扩增片段限制性分析（ARDRA）将随机选择的413个克隆分为24种限制性模式。测定了44个代表性克隆的ITS序列，并使用空位BLAST与GenBank数据库进行比较。系统发育分析表明，ITS序列（相似度71-97%）与茎点霉属、罗也酵母属、马拉色菌属、隐球菌属、柱孢属、霍塔菌属、毕赤酵母属、曲霉属和念珠菌属的序列相似。其余序列与公共数据库中任何已知真菌或真菌序列均无关联。结果表明，含甲烷水合物的深海海洋沉积物中存在多种真菌。这是关于南海含甲烷水合物深海海洋沉积物真菌群落的首次报道。
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