Qu Jia, Kaihui Liu, Ding Xiaowei, Deng Baiwan, Chen Wenqiang, Guo Qinglan, Tian Xinpeng, Zhang Si, Li Wenjun
Wei Sheng Wu Xue Bao. 2014 May 4;54(5):552-62.
We studied species diversity and enzyme activities of fungi from 11 marine sediment samples in the South China Sea.
Salt-tolerant fungi were isolated by a dilution-plate method, and their diversity was studied based on fungal morphology and rDNA ITS (Internal Transcribed Spacer) sequences. Enzyme activities were screened by six special selective media.
A total of 1689 strains of salt-tolerant fungi were isolated. Morphology and ITS sequence analysis identified these fungi to 41 species of 15 described genera, of which Aspergillus sp. and Penicillium sp. were the dominant populations. Studies on enzyme activities of 41 sequenced strains showed that 8 strains produce cellulase, 9 strains produce amylase, 5 strains produce compound enzyme, 16 strains produce protease, 3 strains produce lipase and no strain produce chitonsanase. Acrodontium sp. 8m and Aspergillus sp. 86b produced the most multiple enzymes, while Penicillium sp. 41m produced comparatively higher protease.
There were abundant salt-tolerant fungi from marine sediment samples in the South China Sea, and more strains had enzyme activities.
我们研究了中国南海11个海洋沉积物样本中真菌的物种多样性和酶活性。
采用稀释平板法分离耐盐真菌，并基于真菌形态和rDNA ITS（内转录间隔区）序列研究其多样性。通过六种特殊选择性培养基筛选酶活性。
共分离出1689株耐盐真菌。形态学和ITS序列分析将这些真菌鉴定为15个已描述属的41个物种，其中曲霉属和青霉属为优势种群。对41株测序菌株的酶活性研究表明，8株产生纤维素酶，9株产生淀粉酶，5株产生复合酶，16株产生蛋白酶，3株产生脂肪酶，无菌株产生壳聚糖酶。顶孢霉属8m和曲霉属86b产生的酶种类最多，而青霉属41m产生的蛋白酶相对较高。
中国南海海洋沉积物样本中有丰富的耐盐真菌，且更多菌株具有酶活性。
