Kwon Min-Soo, Seo Young-Jun, Lee Jin-Koo, Lee Han-Kyu, Jung Jun-Sub, Jang Jeong-Eun, Park Soo-Hyun, Suh Hong-Won
Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University, 1 Okcheondong, Chuncheon, Gangwon-Do 200-702, South Korea.
Neurosci Lett. 2008 Jan 17;430(3):258-63. doi: 10.1016/j.neulet.2007.11.006. Epub 2007 Nov 6.
The effect of repeated immobilization stress (RIS) on the expression of interleukin-1beta (IL-1beta) and types of cells that express IL-1beta in hippocampal CA1 region, striatum and paraventricular nucleus (PVN) were investigated in ICR mice. The RIS was induced daily for 2h for 4 consecutive days. In the immunohistochemical study, RIS increased IL-1beta immunoreactivities (IR) in the hippocampal CA1 region and striatum and PVN. The RIS also increased glial fibrillary acidic protein (GFAP) IR and complement receptor type 3 (OX-42) IR in the hippocampal CA1 regions and striatum but not PVN. In confocal immunofluorescence study, the IL-1beta IR increased by RIS were colocalized with only NeuN, but not GFAP or OX-42 in the hippocampal CA1 region, striatum and PVN. Our results indicate that RIS increases IL-1beta IR on neuron, but not astrocyte or microglia in the hippocampal CA1 region, striatum and PVN, suggesting that the IL-1beta IR on neuron may play an important role during RIS. In addition, GFAP and OX-42 increased by RIS may be involved indirectly in playing another role in the hippocampal CA1 region and striatum during RIS.
在ICR小鼠中，研究了重复制动应激（RIS）对海马CA1区、纹状体和室旁核（PVN）中白细胞介素-1β（IL-1β）表达及表达IL-1β的细胞类型的影响。连续4天每天诱导RIS 2小时。在免疫组织化学研究中，RIS增加了海马CA1区、纹状体和PVN中的IL-1β免疫反应性（IR）。RIS还增加了海马CA1区和纹状体中胶质纤维酸性蛋白（GFAP）IR和补体受体3型（OX-42）IR，但PVN中未增加。在共聚焦免疫荧光研究中，RIS增加的IL-1β IR仅与NeuN共定位，而在海马CA1区、纹状体和PVN中不与GFAP或OX-42共定位。我们的结果表明，RIS增加了海马CA1区、纹状体和PVN中神经元上的IL-1β IR，但不增加星形胶质细胞或小胶质细胞上的IL-1β IR，提示神经元上的IL-1β IR可能在RIS过程中起重要作用。此外，RIS增加的GFAP和OX-42可能在RIS期间间接参与海马CA1区和纹状体中的其他作用。
