Huang Xiaoyan, Wang Deyun, Hu Yuanliang, Lu Yu, Guo Zhenhuan, Kong Xiangfeng, Sun Junling
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Biol Macromol. 2008 Mar 1;42(2):166-71. doi: 10.1016/j.ijbiomac.2007.10.019. Epub 2007 Oct 26.
Four kinds of astragalus polysaccharides (APSs), APS(t), APS(40), APS(50) and APS(60), were extracted by water decoction and one-step or stepwise ethanol precipitation methods, and modified by chlorosulfonic acid-pyridine method to obtain four sulfated APSs (sAPSs) (sAPS(t), sAPS(40), sAPS(50), sAPS(60)), respectively. The effects of four sAPSs on cellular infectivity of bursal disease virus (IBDV) were compared by MTT method taking non-modified APS(t) as control. The results showed that modified sAPSs inhibited IBDV to infect CEF significantly in comparison with non-modified APS(t), which indicated that sulfated modification could enhance the antiviral activity of the APS, by which it would be expected to develop a new-type antiviral drug.
采用水煎煮结合一步或分步乙醇沉淀法提取了4种黄芪多糖（APSs），即APS(t)、APS(40)、APS(50)和APS(60)，并通过氯磺酸-吡啶法对其进行修饰，分别得到4种硫酸化黄芪多糖（sAPSs）（sAPS(t)、sAPS(40)、sAPS(50)、sAPS(60)）。以未修饰的APS(t)为对照，采用MTT法比较了4种sAPSs对法氏囊病病毒（IBDV）细胞感染性的影响。结果表明，与未修饰的APS(t)相比，修饰后的sAPSs能显著抑制IBDV感染鸡胚成纤维细胞（CEF），这表明硫酸化修饰可增强APS的抗病毒活性，有望开发出新型抗病毒药物。
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