Garbett Nichola C, Miller James J, Jenson A Bennett, Chaires Jonathan B
James Graham Brown Cancer Center, Department of Medicine, University of Louisville Health Sciences Center, Louisville, KY 40202, USA.
Semin Nephrol. 2007 Nov;27(6):621-6. doi: 10.1016/j.semnephrol.2007.09.004.
The plasma proteome is a complex mixture of more than 3,000 proteins that has routinely been exploited by physicians for clinical diagnostic assays. More recently, the low-abundance region of the proteome has been examined for potential biomarkers of disease. A calorimetric assay has been developed that exploits a new physical basis with which to interrogate the plasma proteome. This article provides a brief overview of the use of the plasma proteome in clinical diagnosis and biomarker discovery and then introduces the new calorimetric assay. Some initial results are reported that indicate the potential clinical utility of the assay.
血浆蛋白质组是由3000多种蛋白质组成的复杂混合物，医生们一直将其用于临床诊断检测。最近，人们开始研究蛋白质组中的低丰度区域，以寻找疾病的潜在生物标志物。一种利用新物理原理来检测血浆蛋白质组的比色测定法已经开发出来。本文简要概述了血浆蛋白质组在临床诊断和生物标志物发现中的应用，然后介绍了这种新的比色测定法。文中还报告了一些初步结果，这些结果表明了该测定法的潜在临床应用价值。
