Kaspárek P, Pantůcek R, Kahánková J, Růzicková V, Doskar J
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 611 37 Brno, Czechia.
Folia Microbiol (Praha). 2007;52(4):331-8. doi: 10.1007/BF02932087.
Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and -negative Staphylococcus species. Mutational changes in the genome of several host-range mutants of phage 812 were identified. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Four mutants (812i, 812b, 812p, 812F3) harbor deletion in the structural gene orf8 that results from a genome rearrangement associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages U16 and phi131. Another intron was discovered in the recA812 gene in these four mutants. An insertion was found in a non-coding region of the restriction fragment PstI-O of three mutants (812b, 812F3, 812g) and phages U16 and phi131. The above results contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
突变使肌尾噬菌体科的多价噬菌体812的宿主范围扩大，在高达95%的金黄色葡萄球菌菌株以及43%的不同凝固酶阳性和阴性葡萄球菌属菌株中均有体现。已鉴定出噬菌体812的几个宿主范围突变体基因组中的突变变化。宿主范围突变体812F1的溶菌酶基因存在缺失，该缺失与内含子切除同时出现。四个突变体（812i、812b、812p、812F3）的结构基因orf8存在缺失，这是由与内含子插入相关的基因组重排导致的。在密切相关的噬菌体U16和phi131的基因组中也检测到了这种重排。在这四个突变体的recA812基因中发现了另一个内含子。在三个突变体（812b、812F3、812g）以及噬菌体U16和phi131的限制性片段PstI-O的非编码区域发现了一个插入。上述结果有助于解释影响多价葡萄球菌噬菌体宿主范围的遗传因素。
