Abiko T, Sekino H
Kidney Research Laboratory, Sendai, Japan.
Chem Pharm Bull (Tokyo). 1991 Oct;39(10):2647-52. doi: 10.1248/cpb.39.2647.
A fragment corresponding to N-terminal octaeicosapeptide of rat parathymosin alpha was synthesized by assembling 5 peptide fragments, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio 1:1) in trifluoroacetic acid in the presence of dimethylselenium. Incubation of impaired T-lymphocytes isolated from uremic patients with the synthetic parathymosin alpha fragment 1-28 showed no immunological restoring effect, but when it was administered together with thymosin alpha 1, it appeared to suppress the restoring effect of the thymosin alpha 1 on the impaired T-lymphocytes of uremic patients.
通过组装5个肽片段合成了与大鼠副胸腺素α N端28肽相对应的片段，随后在二甲基硒存在下，于三氟乙酸中用1 M三氟甲磺酸-苯甲硫醚（摩尔比1:1）进行脱保护。用合成的胸腺素α片段1-28孵育从尿毒症患者中分离出的受损T淋巴细胞，未显示出免疫恢复作用，但当它与胸腺素α1一起给药时，似乎会抑制胸腺素α1对尿毒症患者受损T淋巴细胞的恢复作用。
